The United States of America, As Represented by the Secretary, Department of Health and Human Services

University of California

The University of North Carolina at Charlotte

Bruce Allen Shapiro

Kirill Andreevich Afonin

Mathias D Viard

Luc Jaeger

Eckart Bindewald

Wojciech Kasprzak

Yaroslava Georgievna Yingling

Angelica N Martins

Paul J Zakrevsky

Justin R Halman

Marina A Dobrovolskaia

Eckart H U Bindewald

Arti N Santhanam

Wade W Grabow

Mikhail Kashlev

Maria L Kireeva

Cody Geary

Isil Severcan

Angelica Nascimento Martins

Eckart HU Bindewald

Mathias Denis Viard

Disclosed are nanostructures comprising a ribonucleic acid (RNA) scaffold comprising a hexameric tetrahedral core. The tetrahedral core may comprise four hexameric RNA nanorings linked together. Related pharmaceutical compositions, methods of modulating the expression of a target gene in a mammal, methods of treating or preventing a disease in a mammal, and methods of producing a hexameric tetrahedral RNA nanostructure are also disclosed.

Hexameric tetrahedral RNA nanostructures

The description provides a molecular switch comprising at least two nanoparticles, wherein a first nanoparticle comprises DNA and/or RNA oligonucleotides, and a second nanoparticle which is complementary to the first nanoparticle comprises reverse complementary DNA and/or RNA oligonucleotides of the first nanoparticle; wherein the complementary nanoparticles interact under physiological conditions leading to thermodynamically driven conformational changes in the first and second nanoparticles leading to their re-association to release one or more duplexes comprising said DNA and/or RNA oligonucleotides and the reverse complementary DNA and/or RNA oligonucleotides, and wherein the nanoparticles are not rings and have no single stranded toeholds.

Functionally-interdependent shape switching nucleic acid nanoparticles

The instant invention provides RNA nanocubes, DNA nanocubes and R/DNA chimeric nanocubes comprising one or more functionalities. The multifunctional RNA nanocubes are suitable for therapeutic or diagnostic use in a number of diseases or disorders.

Triggering RNA interference with RNA-DNA and DNA-RNA nanoparticles

The instant invention provides RNA nanoparticles and R/DNA chimeric nanoparticles comprising one or more functionalities. The multifunctional RNA nanoparticles are suitable for therapeutic or diagnostic use in a number of diseases or disorders.

Multifunctional RNA nanoparticles and methods of use

The instant invention provides polyvalent RNA nanoparticles comprising RNA motifs as building blocks that can form RNA nanotubes. The polyvalent RNA nanoparticles are suitable for therapeutic or diagnostic use in a number of diseases or disorders.

RNA nanoparticles and nanotubes

A method is provided for generating RNA nanoparticles having modified nucleotides and/or having increased nuclease resistance where the RNA nanoparticles are formed cotranscriptionally by T7 RNA polymerase in the presence of manganese ions.

Co-transcriptional assembly of modified RNA nanoparticles

Auto-recognizing therapeutic R/DNA chimeric nanoparticles (R/DNA NP) are described that are pairs of DNA/RNA hybrids where the DNA molecules have complementary toehold sequences that promote the re-association of the R/DNA NPs when mixed resulting in the formation of RNA/RNA duplexes that act as siRNAs.

Auto-recognizing therapeutic RNA/DNA chimeric nanoparticles (NP)

Growing community of inventors

Gaithersburg, MD, United States of America

Bruce Allen Shapiro