Carnegie Mellon University

Other

University of Pittsburgh

Bruce Alan Armitage

Danith H Ly

Munira F Fouz

Krzysztof A Matyjaszewski

Hans-Georg Batz

Henrik Frydenlund Hansen

Troels Koch

Henrik Orum

Saadyah Averick

Subha Ranjan Das

Gary B Schuster

Patricia L Opresko

Veronica Hinman

Nathaniel Shank

Provided herein are methods of detecting target analytes, such as nucleic acids, for example microRNAs using an enhanced Tyramide Signal Amplification (TSA) method that employs probes tagged with tyramide-binding groups to amplify the effects of the TSA. The accessibility of the tyramide-binding groups, such as hydroxyphenyl groups, provides for large improvements in signal due to faster reaction with the radicals. The present invention further includes the application of the assay for detecting specific microRNAs.

Enhanced biomolecule detection assays based on tyramide signal amplification and gammaPNA probes

A composition includes a polymer including extending chains, side chains, or branches. One (or more) of a plurality of a first strand of nucleic acid is attached to each of a plurality of the side chains. One (or more) of a plurality of a second strand of nucleic acid, which is complementary to the first strand of nucleic acid, is complexed to each of the plurality of the first strand of nucleic acid to form a double strand of nucleic acid on each of the plurality of the side chains. At least one fluorescent compound is associated with the double strand of nucleic acid on each of the plurality of the side chains.

Nucleic acid-polymer conjugates for bright fluorescent tags

A category of &#x3b3;PNA miniprobes and chimeric &#x3b3;PNA probes is especially useful for detecting RNA and telomeric DNA in a cell sample. In particular, the probes can be used to deliver fluorescent dyes to the telomeres, allowing direct visualization of telomeres in cells.

Gamma-PNA miniprobes for fluorescent labeling

New electron transfer moiety labeled nucleic acid analogue probes are provided that can be used in methods for determining nucleic acids in a sample. The new probes can be prepared using novel monomer subunits in a chemical synthesis route. The nucleic acids can be determined by binding the probe molecules to the nucleic acid and inducing electron transfer within the complex formed. The occurrence of the electron transfer is determined as a measure of the nucleic acid.

Method for determining a nucleic acid

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Pittsburgh, PA, United States of America

Bruce Alan Armitage