Other

Incyte Pharmaceuticals, Inc.

Bruce A Wang

Donald G Payan

Joseph R Fisher

Scott Wang

Alicia Chung

Methods are provided for quantitative gene expression analysis. In the subject methods, end-labeled target nucleic acid is contacted with an array of probe molecules stably associated with the surface of a solid support under hybridization conditions sufficient to produce a hybridization pattern. The resultant hybridization pattern can be used to obtain a quantitative information about the genetic profile of the end-labeled target nucleic acid sample, as well as the physiological source from which it is derived. As such, the subject methods find use in a variety of applications.

Quantitative microarray hybridizaton assays

Cyclophilin-60 proteins, nucleic acids, and monoclonal antibodies are provided.

Nuclear cyclophilin

Methods of enzymatically producing unbiased amounts of nucleic acid from mRNA are provided. In the subject methods, a sample of mRNA is converted to ds cDNA using a primer containing an RNA polymerase site and at least one priming site. The resultant ds cDNA is then asymmetrically amplified with captureable primer to produce captureable cDNA which is then converted to captured ds cDNA. The resultant ds cDNA may then be used in a number of different applications, such as in the preparation of amplified amounts of aRNA, in the preparation of cDNA probes, and the like.

Method for unbiased mRNA amplification

This invention provides a method of assembling a liquid applicator structure having a static mixing member with a input and an output end and a nozzle member disposed at the output end of the mixing member, comprising the steps of providing a static mixing member; providing a nozzle member; depositing a predetermined amount of an adhesive on the output end of the static mixing member; connecting the nozzle member to the output end of the static mixing member; and applying a retention structure over a predetermined segment of the mixing member and the nozzle member. Also provided is an applicator apparatus for dispensing viscous livid compounds, and for use with supply and drive means for the compounds, comprising: a static mixing member having an input end and an output end; a nozzle member connected to the output end of the static mixing member; a retention structure disposed over and coupling predetermined segments of the mixing member and the nozzle member; and wherein the static mixing member and the nozzle member are adhesively bonded together.

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Houlton, WI, United States of America

Bruce A Wang