Carnegie Mellon University

Brigitte F Schmidt

Alan S Waggoner

Marcel Pierre Bruchez

Jonathan W Jarvik

Christopher S Szent-Gyorgyi

Peter B Berget

Lauren A Ernst

Subhasish K Chakraborty

James Fitzpatrick

Chris Szent-Gyorgyi

Chris Szent-Gyorgi

Biosensor comprising an activatable acceptor fluorogen linked via a linker to a donor which transfers energy to the fluorogen on detecting an analyte wherein the fluorogen component reacts and a 100 fold increase in intensity results when the fluorogen interacts non-covalently with an activator e.g. fluorogen activator peptide.

Targeted probes of cellular physiology

Provided are biosensors, compositions comprising biosensors, and methods of using biosensors in living cells and organisms. The biosensors are able to be selectively targeted to certain regions or structures within a cell. The biosensors may provide a signal when the biosensor is targeted and/or in response to a property of the cell or organism such as membrane potential, ion concentration or enzyme activity.

Optical biosensors

The present invention presents designs for high extinction quenched 'dyedrons' that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron.

Quenched dendrimeric dyes for florescence detection

Growing community of inventors

Pittsburgh, PA, United States of America

Brigitte F Schmidt