Cargill Inc.

Briana Kozlowicz

Sara C McFarlan

Gregory Michael Poynter

Ana Negrete-Raymond

Erin Kathleen Marasco

Beth M Mastel

Amit Vas

Kenneth R Finley

Chenfeng Lu

Sara C Mcfarlan

A system for editing of a target sequence at a locus of a host cell is disclosed. The system has a nucleic acid molecule comprising a nucleic acid segment comprising a targeting RNA sequence and an RNA segment that binds a protein. The system also has a nucleic acid molecule comprising a nucleic acid segment encoding a polypeptide with endonuclease activity fused to a protein that binds the RNA segment. The system also comprises a double stranded DNA molecule comprising DNA comprising at least one nucleotide sequence that is capable of binding to the target sequence at the locus.

Genome editing system

The present invention relates to a genetically engineered yeast (e.g., Crabtree negative) that express a heterologous xylose isomerase. In a fermentation method the engineered yeast are capable of producing a bioproduct, such as ethanol, in a fermentation medium that includes xylose. Desirable bioproduct titers can be achieved when materials such as acetate are present in the fermentation medium.

Xylose isomerase-modified yeast strains and methods for bioproduct production

Growing community of inventors

Hopkins, MN, United States of America

Briana Kozlowicz