Massachusetts Institute of Technology

University of Alberta

University of Pennsylvania

Revolution Medicines, Inc.

Warp Drive Bio, Inc.

Harvard College

Georgia Tech Research Corporation

Princeton University

Brian Yichiun Chow

Edward Stuart Boyden

Nathan Klapoetke

Yongku Peter Cho

Joseph M Jacobson

David W Mosley

Peter A Carr

Christopher Emig

Gane Ka-Shu Wong

Gregory Lawrence Verdine

David M Armistead

David W White

Mathew Edward Sowa

Deborah Palestrant

Chris K Varma

Amy S Chuong

Xue Han

Bohdana Marie Discher

Peter Leslie Dutton

Christopher Moser

Goutham Kodali

Molly Marie Sheehan

Joshua Mancini

Michael Magaraci

Nathan M Ennist

Adam Ezra Cohen

Manu Prakash

Daniel Hochbaum

Jose L Avalos

Suhasa Bangalore Kodandaramaiah

Giovanni Talei Franzesi

David Gonzalez-Martinez

Saachi Datta

William Benman

Lukasz Bugaj

Gane K S Wong

Crag Richard Forest

Jaebum Joo

Provided herein is a stimulation and measurement device. The stimulation and measurement device includes a microwell plate having a top side, a bottom side, and at least one well extending from the top side towards the bottom side; a first assembly removably positioned on the top side of the microwell plate; a second assembly removably positioned on the bottom side of the microwell plate; and a microcontroller. The first assembly includes a first printed circuit board having at least one pair of stimulating light emitting diodes (LEDs) positioned thereon to align with one of the wells. The second assembly includes a second printed circuit board having at least one photodiode positioned thereon to align with one of the pairs of stimulating LEDs, and at least one excitation LED paired with each of the photodiodes. The microcontroller is configured to control the stimulating LED(s), the photodiode(s), and the excitation LED(s).

Photostimulation device and methods of using the same

The invention, in some aspects relates to compositions and methods for altering cell activity and function and the introduction and use of light-activated ion channels.

Channelrhodopsins for optical control of cells

De novo artificial protein based reporters that may be expressed in eukaryotic (e.g., mammalian) cells and methods of using the same are provided herein.

Artificial proteins and compositions and methods thereof

Disclosed herein are methods for identifying novel drug candidates.

Identifying new therapeutic agents

In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3' or 5&#x2032; protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3&#x2032; or 5&#x2032; associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.

Methods for high fidelity production of long nucleic acid molecules

CsChrimson light-activated ion channel polypeptides, their encoding polynucleotides, and variants thereof are provided. Methods of introducing and using CsChrimson light activated ion channels and variants thereof for to alter cell activity and function are also provided.

Channelrhodopsin variants and uses thereof

The invention, in some aspects relates to light-activated ion channel polypeptides and encoding nucleic acids and also relates in part to compositions comprising light-activated ion channel polypeptides and methods using light-activated ion channel polypeptides to alter cell activity and function.

Blue-light-activated ion channel polypeptides and uses thereof

Growing community of inventors

Cambridge, MA, United States of America

Brian Yichiun Chow