University of Utah Research Foundation

Yale University

University of Maine System

The Jackson Laboratory

Brian Thomas Bennett

Joerg Bewersdorf

Erik Jorgensen

Sam Hess

Travis J Gould

Mudalige Siyath Gunewardene

A system () and method for creating three dimensional images using probe molecules is disclosed and described. A sample is mounted on a stage (). The sample has a plurality of probe molecules. The sample is illuminated with light, causing the probe molecules to luminesce. The probe luminescence can be split into at least four paths corresponding to at least four detection planes corresponding to object planes in the sample. The at least four detection planes are detected via a camera (). Object planes in corresponding recorded regions of interest are recorded in the camera (). A signal from the regions of interest is combined into a three dimensional image.

Microscopy system and method for creating three dimensional images using probe molecules

A primary beam splitter () of an optical apparatus () can be used to split an incident light beam () into a primary plurality of light beams and to direct a first beam therefrom in a first direction and a second beam therefrom in a second direction orthogonal to the first direction. Secondary beam splitters () positioned in beam paths of the first and second beams can be used to split the first and second beams of the primary plurality of light beams into a secondary plurality of light beams () and to split the same into a tertiary plurality of light beams (). A primary plurality of beam reflectors () can be positioned and used to redirect the secondary and tertiary plurality of light beams toward a common detector ().

Beam splitter module having a secondary beam splitter for providing orthogonal light beams and parallel light beams

Growing community of inventors

Park City, UT, United States of America

Brian Thomas Bennett