Battelle Memorial Institute

Brian S Hooker

Johnway Gao

Daniel B Anderson

Rodney S Skeen

Ziyu Dai

Lifang Shi

The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strainThe isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Isolated yeast promoter sequence and a method of regulated heterologous expression

The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.

Isolation and characterization of the genomic DNA clones of ribosomal protein gene L25 in tobacco

The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Method for using a yeast alpha-amylase promoter

Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.

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Kennewick, WA, United States of America

Brian S Hooker