Corning Incorporated

Brian L Webb

Ye Fang

Yulong Hong

Fang Lai

Laurent A G Picard

Ann MeeJin Ferrie

Jinlin Peng

Joydeep Lahiri

John Christopher Mauro

Po Ki Yuen

Valerie Claudine Lacarriere

Bernice I Feuer

Srikanth Raghavan

Alexander Mikhailovich Efremov

Sadashiva K Pai

David M Root

Lin He

Keith A Horn

Alain R E Carre

Youchun Shi

Mircea Despa

Michael Donavon Brady

Alain Re Carre

James B Stamatoff

Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

Method and device for protein delivery into cells

A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.

Porous substrate plates and the use thereof

The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.

Membrane arrays and methods of manufacture

Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5'-diphosphate (GDP) salt, and/or an anti-oxidant reagent.

Assay solution compositions and methods for GPCR arrays

Systems, methods and kits that utilize uniquely coded microparticles for performing protein assays are provided. The uniquely coded microparticles are used as a substrate for reverse protein delivery into cells. The microparticles and methods offer the possibility of studying the biological functions of either a single protein of interest in multiple cell types per assay or multiple proteins in a single cell type.

Reverse protein delivery into cells on coded microparticles

A biological assay device for use in molecular biology, pharmaceutical research, genomic analysis, combinatorial chemistry, and in the general field of the analysis of molecules that may be deposited on supports of various kinds is provided. Specifically, the present invention includes a fluidic or microfluidic device, which integrates fluidic capability into existing multi-well plates of standard configuration, for performing either single or continuous fluidic manipulations in a high-throughout format. Methods for the use and manufacture of these devices are also provided.

Microcolumn-based, high-throughput microfluidic device

A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.

Microcolumn-platform based array for high-throughput analysis

A cell transfection apparatus and methods for making and using the cell transfection apparatus are described. The cell transfection apparatus has a surface on which there is printed at different locations one or more foreign biomolecules (e.g., DNA, RNA, oligonucleotides, nucleotides). The foreign biomolecules can be printed at the same time. The surface is then covered with a transfection reagent which is incubated and removed from the surface before cells in a cell growth media are placed on the surface. The surface is configured such that the cells which become transfected with one or more foreign biomolecules are segregated from the cells which fail to become transfected with one or more foreign biomolecules. There are disclosed two embodiments of the cell transfection apparatus.

Cell transfection apparatus and methods for making and using the cell transfection apparatus

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Painted Post, NY, United States of America

Brian L Webb