Archerdx, LLC

Watchmaker Genomics, Inc.

Brian Kudlow

Jason Myers

Joshua Stahl

Brady Culver

Bjarne Faurholm

Ross Wadsworth

Martin Ranik

Travis J Sanders

Craig Marshall

Clara Ross

Lindsay Peterkin

Eric Van Der Walt

Jens Eberlein

Julie Walker

Disclosed herein are methods and compositions for normalizing polynucleotide concentration. Normalizing may be accomplished using polynucleotide binding proteins (e.g., catalytically inactive CRISPR protein or catalytically inactive Argonaute proteins). The polynucleotide binding proteins may bind to the adapter sequences of a target polynucleotide. Thus, adding the same amount of a polynucleotide binding proteins to different samples and then extracting the polynucleotide protein is shown herein to extract similar amounts of target polynucleotides from the different samples.

Methods and compositions for sequencing library normalization

Aspects of the technology disclosed herein relate to methods of preparing and analyzing nucleic acids. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.

Methods of nucleic acid sample preparation

The present invention provides MMLV reverse transcriptase enzymes with increased thermal stability as compared with wild type MMLV and AMV reverse transcriptases. The improved thermal stability allows for reverse transcription of RNA to cDNA at temperatures above 37&#xb0; C., thereby reducing error rates introduced during cDNA synthesis. As a result, the reverse transcriptases of the invention allow for increased accuracy in the determination of transcriptomes of living organisms.

Reverse transcriptase variants

Aspects of the technology disclosed herein relate to methods of preparing and analyzing nucleic acids, e.g, cfDNA. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.

Methods of nucleic acid sample preparation for analysis of DNA

Aspects of the technology disclosed herein relate to methods of preparing and analyzing nucleic acids, e.g., nucleic acids encoding immune receptors and immunoglobulins. In some embodiments, methods for preparing nucleic acids for sequence analysis (e.g., using next-generation sequencing) are provided herein.

Methods of nucleic acid sample preparation for immune repertoire sequencing

Methods of nucleic acid sample preparation for analysis of cell-free DNA

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Boulder, CO, United States of America

Brian Kudlow