University of Illinois

Arizona State University

Brian Kay

Kevin Gorman

Joshua LaBaer

Ji Qiu

Jie Wang

Michael Kierny

Kritika Pershad

Renhua Huang

Methods for screening of affinity reagents for many target proteins of interest simultaneously. Arrayed targets (e.g., peptide, protein, RNA, cell, etc.) are used in affinity selection experiments to reduce the amount of target needed and to improve the throughput of discovering recombinant affinity reagents to a large collection of targets.

Generating recombinant affinity reagents with arrayed targets

A combined Kunkel mutagenesis and phage-display method for producing bivalent binding agents is provided.

Method for generating high affinity, bivalent binding agents for sandwich assays

A combined ribosome-display and phage-display method and kit for carrying out the method are provided. The method includes screening a ribosome-display library of binding agents to identify binding agents that interact with one or more target molecules of interest, converting the RNA encoding the binding agents to a phage-display format by amplification and primer extension, and the screening the phage-display library to enrich for binding agents that interact with one or more target molecules of interest.

Method and kit for generating high affinity binding agents

This invention relates to the use of protein scaffolds for producing affinity reagents that are polypeptides that specifically bind to phosphopeptides.

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Chicago, IL, United States of America

Brian Kay