Cargill Inc.

Bioamber Inc.

Novozymes A/s

Natureworks LLC

Rush, Brian J.

Beacom, Daniel R.

Reeder, David H.

Kolstad, Jeffrey L.

Brian Jeffrey Rush

Gregory Michael Poynter

Arlene M Fosmer

Thomas W McMullin

Beth M Mastel

Jeanette M Huryta

Debbie Sue Yaver

Holly J Jessen

Aristos Aristidou

Alan Berry

Kevin T Watts

Michael Glenn Catlett

Michelle Barnhart

Vernon L McIntosh, Jr

Pim Van Hoek

Peter Alan Jauert

Jeffrey John Kolstad

Christopher K Miller

David Hiram Reeder

Jon Veldhouse

Daniel Roger Beacom

Catherine Asleson Dundon

Kari Koivuranta

Benjamin Matthew Hause

Chris Miller

Kenneth R Finley

Kevin Roberg-Perez

Keith M Brady

Brynne Stanton

Joshua Dunn

Pirkko Suominen

Ana Negrete-Raymond

Amit Vas

Thomas William McMULLIN

Pirrko Suominen

Genfeng Lu

Jeffrey L Kolstad

Michelle Barnhardt

Genetically modified yeast having a heterologous sugar transporter that is capable of transporting a non-glucose sugar such as maltulose, are described. The heterologous sugar transporter can be a protein according to, or that has similarity to, SEQ ID NO: 44. Fermentation methods using enzymatically treated starch where the yeast are able to consume the non-glucose sugars, are also described. The engineered yeast can be useful for producing desired bioproducts such as high ethanol, with low amounts of residual sugars in the medium.

Sugar transporter-modified yeast strains and methods for bioproduct production

The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP.

Compositions and methods for 3-hydroxypropionic acid production

The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32° C. or greater.

Glucoamylase engineered yeast and fermentation methods

The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume glucose and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).

Genetically modified lactate-consuming yeasts and fermentation processes using such genetically modified yeasts

Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P+) transhydrogenase enzyme

The present invention relates to genetically engineered yeasts having a heterologous trehalase gene and fermentation processes for using such yeasts. The yeasts can express trehalase in a quantity sufficient to convert significant amounts of trehalose to glucose, thereby improving the yield of the product in a fermentation, and/or reducing or eliminating the need to add exogenous trehalase to the fermentation. The yeasts can also include other heterologous genes for expressing enzymes useful for improving yield and/or for reducing or eliminating the need to add exogenous enzymes to the fermentation.

Genetically modified trehalose-expressing yeasts and fermentation processes using such genetically modified yeasts

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the generaor. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate.

Compositions and methods for succinate production

The invention is directed to an engineered yeast including an exogenous nucleic acid encoding a glucoamylase comprising SEQ ID NO:1 and SEQ ID NO:4, or a variant thereof. The engineered yeast are able to provide glucoamylase into a fermentation media and cause degradation of starch material generating glucose for fermentation to a desired bioproduct, such as ethanol. High titers of bioproduct (e.g., 70 g/kg of ethanol) can be achieved, along with low residual glucose levels. Further the yeast exhibit good growth and bioproduct product at temperatures of 32&#xb0; C. or greater.

The present invention relates to genetically modified yeasts that can use lactate as a carbon source to produce a fermentation product. In one aspect, the yeasts can consume gluconse and lactate simultaneously to produce ethanol. In one aspect, the genetically modified yeast is transformed to include a monocarboxylic/monocarboxylate transporter. In one aspect, the yeast can include one or more heterologous genes encoding lactate dehydrogenase (cytochrome) (EC 1.1.2.3 and/or 1.1.2.4).

Genetically modified trehalase-expressing yeasts and fermentation processes using such genetically modified yeasts

Yeast cells having reductive TCA pathway from pyruvate to succinate and overexpressing an exogenous NAD(P)+ transhydrogenase enzyme

Fermentation process using yeast cells having disrupted pathway from dihydroxyacetone phosphate to glycerol

The present invention relates to a genetically engineered yeast capable of manufacturing a fermentation product using sucrose as a fermentation substrate, and fermentation processes using such a yeast. In some embodiments, the fermentation product is ethanol.

Genetically modified yeasts and fermentation processes using genetically modified yeasts

Growing community of inventors

Minneapolis, MN, United States of America

Brian Jeffrey Rush