Butamax Advanced Biofuels LLC

Butamax (tm) Advanced Biofuels LLC

The Procter Gamble Company

Brian James Paul

Rick Weizhang Ye

Dennis Flint

Steven Cary Rothman

Wonchul Suh

Lori Ann Maggio-Hall

Mark Scott Payne

Daniel P O'Keefe

Hongxian He

Larry Cameron Anthony

Arthur Leo Kruckeberg

Sandra W Ramer

Michael Dauner

Daniel P Okeefe

Michelle Jackson

Robert A Larossa

Jessica McElvain

Joseph Frederich Tuminello

Manasi Bhate

Patricia Tran

Hon Kit Chan

Jonathan Lassila

Katarzyna Bell-Rusiewicz

An automatic dishwashing cleaning composition comprising a variant α-amylase wherein the variant α-amylase comprises amino acid substitution(s) selected from the group consisting of:

Automatic dishwashing detergent composition comprising an amylase

The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe&#x2014;S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe&#x2014;S cluster biosynthesis pathway in a host cell.

Activity of Fe&#x2014;S cluster requiring proteins

Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

DHAD variants for butanol production

Activity of Fe-S cluster requiring proteins

Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.

Keto-isovalerate decarboxylase enzymes and methods of use thereof

The invention relates to suitable screening strategies for evaluating various candidate promoters for differential gene expression during the propagation and production phases of a fermentation process. The invention also relates to recombinant host cells that comprise identified promoter nucleic acid sequences and methods for producing fermentation products employing the same.

Genetic switches for butanol production

An engineering method was developed to allow genetic modification and isolation of lactic acid bacteria cells that lack lactate dehydrogenase and acetolactate decarboxylase activities. In cells with these modifications and an isobutanol biosynthetic pathway, improved production of isobutanol was observed.

Flux to acetolactate-derived products in lactic acid bacteria

A high flux of metabolites from pyruvate to 2,3-butanediol inwas achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.

Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria

Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 &#x3bc;mol minmg. LAB cells with even higher activities of 0.2 to 0.6 &#x3bc;mol minmgof DHAD activity were obtained. These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.

Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria

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Wilmington, DE, United States of America

Brian James Paul