Life Technologies Corporation

Invitrogen Corporation

Brian J Schmidt

Jhy-Jhu Lin

Deb K Chatterjee

Mary C Longo

Fredric R Bloom

Michael D Smith

A John Hughes, Jr

Ilse I Blumentals

Roger S Lasken

Frederic R Bloom

The present invention provides novel rapidly growing microorganisms and methods for their use in cloning or subcloning nucleic acid molecules. The rapid growing microorganisms of the present invention form colonies more rapidly than microorganisms typically used in molecular biology and thus provide a significant improvement in in vitro cloning methods used extensively in molecular biology. The invention also relates to kits and compositions used in the methods of the invention.

Rapid growing microorganisms for biotechnology applications

The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.

Thermophilic DNA polymerase

The present invention is directed to recombinant hosts which contain and express the AluI Type-II restriction endonuclease gene. The present invention is also directed to vectors or DNA molecules which contain the gene, and to methods of producing the AluI enzyme. One source of the enzyme is Arthrobacter luteus, although other microorganisms may be used to isolate restriction endonuclease isoschizomers of the invention.

Cloning and expression of AluI restriction endonuclease

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Rockville, MD, United States of America

Brian J Schmidt