Government of the United States of America, as Represented by the Secretary of Commerce

Sol Inc.

Brian J Nablo

Darwin R Reyes-Hernandez

Jong Muk Lee

The present disclosure relates to a real-time quantification method of cell viability through a supravital dye uptake using a lens-free imaging system. The method includes a step of incubating a sample cell in a cell culture medium, steps of detecting light penetrating the cell culture medium and identifying a boundary region of the sample cell at a preset time interval based on the detected light, a step of staining the incubated sample cell with the supravital dye, a step of detecting intensity of light penetrating the cell culture medium at a preset time interval, a step of calculating absorbance of the sample cell included in the cell culture medium at a preset time interval based on the boundary region and the detected intensity of light and a step of analyzing a viability of the sample cell based on the calculated absorbance.

Real-time quantification method of cell viability through supravital dye uptake using lens-free imaging system

A dual dielectropheretic article for monitoring cell migration includes: a membrane to selectively migrate a plurality of cells across the membrane, the membrane including: a first surface to receive the cells; a second surface opposed to the first surface; and a plurality of communication paths disposed in the membrane to provide the selective migration of the cells across the membrane from the first surface to the second surface; a first electrode disposed on the first surface to: provide an electric field for dielectrophoresis of the cells at the first surface; and provide a first potential for monitoring an impedance at the first surface; and a third electrode disposed on the second surface to: provide an electric field for dielectrophoresis of the cells at the second surface; and provide a third potential for monitoring an impedance at the second surface.

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Rockville, MD, United States of America

Brian J Nablo