Great Basin Scientific Corporation

Fluoresentric, Inc.

Brian Hicke

Robert Delmar Jenison

Brian E Caplin

Madeline West

Bryson Green

Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the the rpoB gene.

Methods of isothermal amplification using blocked primers

Methods for rapidly detecting clinically relevant mutations in the infectious genome of an agent are disclosed. The methods include use of a novel target and temperature dependent RNase H mediated cleavage of blocked DNA primers to initiate isothermal helicase-dependent amplification of a target sequence such as a sequence in the rpoB gene.

Growing community of inventors

Snyderville, UT, United States of America

Brian Hicke