Somagenics, Inc.

Other

Realseq Biosciences, Inc.

Integrated Dna Technologies, Inc.

Brian H Johnston

Anne Dallas

Heini Ilves

Sergei A Kazakov

Qing Ge

Pavan Kumar

Mark Aaron Behlke

Roger Louis Kaspar

Attila A Seyhan

Alexander V Vlassov

Sergio Barberan-Soler

Sumedha Jayasena

Catharina Casper-Lindley

Craig Van Kooten

Disclosed herein are methods and compositions for the detection of small RNAs in a sample. The methods and compositions disclosed herein may be used for preparing sequencing libraries of the small RNAs, fragments of RNAs and DNAs.

Methods of library construction for polynucleotide sequencing

Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing.

Methods and compositions of short small hairpin RNAs and microRNAs for wound healing

Aspects of the present invention include the production and use of chemically modified RNAi agents (e.g., shRNAs) in gene silencing applications. The chemically modified RNAi agents disclosed herein have reduced immunostimulatory activity, increased serum stability, or both, as compared to a corresponding RNAi agent not having the chemical modification. Compositions containing chemically modified RNAi agents according to aspects of the present invention (including pharmaceutical compositions) and kits containing the same are also provided.

Chemical modification of short small hairpin RNAs for inhibition of gene expression

Methods and compositions of short small hairpin RNAS and microRNAS for wound healing

Currently, the circularization of small RNAs is broadly regarded as an obstacle in ligation-related assays and explicitly avoided while short lengths of linear RNA targets is broadly recognized as a factor limiting use of conventional primers in PCR-related assays. In contrast, the disclosed invention capitalizes on circularization of small RNA targets or their conjugates with oligonucleotide adapters. The circular RNA templates provide amplification of the target sequences via synthesis of multimer nucleic acids that can be either labeled for direct detection or subjected to PCR amplification and detection. Structure of small circular RNAs and corresponding multimeric nucleic acids provide certain advantages over current methods including flexibility in design of conventional RT and PCR primers as well as use of 5'-overlapping dimer-primers for efficient and sequence-specific amplification of short target sequences. Our invention also reduces number of steps and reagents while increasing sensitivity and accuracy of detection of small RNAs with both 2&#x2032;OH and 2&#x2032;-OMe at their 3&#x2032; ends. Our invention increase sensitivity and specificity of detection of microRNAs and other small RNAs with both 2&#x2032;OH and 2&#x2032;-OMe at their 3&#x2032; ends while allowing us to distinguish these two forms from each other.

Methods and compositions for detection of small RNAs

Disclosed herein are compositions and methods for the processing, amplification and detection of polynucleotides using target-specific oligonucleotides (TSOs). Hybridization of TSOs to target polynucleotides guides target processing into and purification of small target fragments that then can be amplified and detected with high sensitivity and reproducibility. The method is specifically beneficial for highly degraded polynucleotides found in biological samples.

Methods and compositions for detecting polynucleotides and fragments thereof

Methods and compositions for detection of small RNAS

Methods, compositions, and kits that include small hairpin RNA (shRNA) useful for inhibition of gene expression, such as viral-mediated gene expression, are described.

Short hairpin RNAs for inhibition of gene expression

The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) that are useful for inhibition of viral-mediated gene expression. Small interfering RNAs as described herein can be used in methods of treatment of HCV infection. ShRNA and siRNA constructs targetING the internal ribosome entry site (IRES) sequence of HCV are described.

Growing community of inventors

Scotts Valley, CA, United States of America

Brian H Johnston