Response Biomedical Corporation

Brian G Richards

Paul C Harris

Donald Elliott Brooks

Jonathan G Tippett

Whalley K Fong

Zongcen Charles Xie

Andrew D Olal

Methods for measuring the amount of two or more analytes of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods involve determining a ratio of a detected amount of a single analyte of interest, to the sum of a detected amount of each of the analytes of interest plus a detected amount of a control, wherein the amount of each analyte of interest is directly or inversely related to the ratio for each analyte of interest.

Multiple analyte immunoassay

A sample testing apparatus () for use in measuring an analyte in a sample () is disclosed. The apparatus includes a sample well () for receiving the sample. A solid analyte binding agent () is disposed in the sample well. The apparatus further includes a mixing device () for mixing the sample with the solid analyte binding agent and a valve (). The valve is configurable between a closed position for impeding flow of the sample from the sample well and an open position for releasing the sample from the sample well. The apparatus additionally includes a membrane () for receiving the sample once released from the sample well, the membrane having a capture reagent () adapted to interact with at least one of the analyte, the analyte binding agent, or a complex thereof, to aid in determining the amount of the analyte in the sample.

Method and apparatus of quantitative assays

Methods for quantitatively measuring the amount of an analyte of interest in a fluid sample are disclosed. The methods involve providing a membrane having an application point, a detection zone, and a contact region, where the contact region is between the application point and the detection zone, and has test particles and internal control particles imbedded within it; contacting the application point with the fluid sample; maintaining the membrane under conditions sufficient to allow fluid to transport analyte by capillary action to the contact region, where the analyte binds to the test particles; further maintaining the membrane under conditions sufficient to allow fluid to transport analyte-bound test particles and internal control particles to the detection zone, where they interact with a detection reagent; and detecting the amount of test particles and the amount of internal control particles in the detection zone. The amount of analyte in the fluid sample is related to a corrected test particle amount, which can be determined, for example, as the difference between the amount of test particles and the amount of internal control particles in the detection zone. Alternatively, the fluid sample can be contacted with the detection zone of the apparatus, and the test particles are mobilized by addition of fluid to the application point. Other methods involve providing a solid phase reactant for an enzyme-linked immunosorbent assay, and contacting the solid phase reactant with a solution containing an initial detection antibody and an internal control antibody. The amount of analyte in the fluid sample is related to a corrected amount of initial detection antibody, which can be determined, for example, as the difference between the amount of initial detection antibody and the amount of internal control antibody on the solid-phase reactant.

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Vancouver, Canada

Brian G Richards