Wisconsin Alumni Research Foundation

Other

University of British Columbia

Brian Fox

Craig A Bingman

John Ralph

Emily Beebe

Steven D Karlen

Rebecca Anne Smith

Hoon Y Kim

Cameron Robert Currie

Taichi Takasuka

Timothy James Donohue

Daniel Noguera

Wayne S Kontur

Yong Chang

Daniel Leo Gall

Adam Joel Book

Shawn D Mansfield

Robert Joseph Stankey

Paul G Blommel

Gary A Wesenberg

Pablo Sobrado

Jeffrey A Haas

Heather Mackay

Yaseen Mottiar

Faride Unda

Christopher M Bianchetti

Nathaniel L Elsen

The present invention relates to a system and method for providing adaptive digital therapy, enhancing the emotional well-being of users through the analysis and translation of expressive and physiological states. Aspects of the invention are directed to identifying the neuroplastic window via user inputs, with or without the aid of psychedelics. This invention is particularly suited for supporting psychoactive therapies involving compounds such as 5-HT2A agonists, dissociatives, and empathogens, as well as applications beyond psychoactive treatments. By utilizing multi-modal deep learning methods, the system captures a wide array of user inputs, including text, speech, vision, symbols, and brain waves, to form a dynamic user representation. This representation is then used to provide real-time, tailored generative communications informed by established psychological therapeutic frameworks. The invention features a closed-loop feedback system through an access module, enabling continuous data relay, patient response metric derivation, and transmission of emotional optimization communications. The system also introduces novel communication methods, including symbolic language input, to articulate complex experiences and extends its capabilities to include natural emotional states, broadening its therapeutic applicability.

Adaptive digital therapy system with real-time emotional state analysis and symbolic language communication

The invention is directed to BAHD acyltransferase enzymes, nucleic acids encoding BAHD acyltransferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of BAHD acyltransferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.

BAHD acyltransferases

The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.

Feruloyl-CoA:monolignol transferases

The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.

P-coumaroyl-CoA:monolignol transferases

Enzymes for depolymerizing lignin. The enzymes include dehydrogenases, &#x3b2;-etherases, and glutathione lyases. The dehydrogenases can comprise one or more or LigD, LigO, LigN, and LigL. The &#x3b2;-etherases can comprise one or more of LigE, LigF, LigP, and BaeA. The glutathione lyases can comprise any one or more of LigG and a number of non-stereospecific, optionally recombinant glutathione lyases derived fromsp. SYK-6,, and other microorganisms. The enzymes can be combined in compositions and/or used in methods of processing lignin or other aromatic compounds in vitro.

In vitro methods of chemical conversion using non-stereospecific glutathione lyases

In vitro methods for processing lignin and other aromatic compounds

A heterologous gene cassette useful for creatingspecies with enhanced capability of growing on a cellulosic polysaccharide substrate, wherein the cassette comprises at least two members of the following categories: a) a GH6 gene, b) an AA10 gene, c) a GH48 gene, d) a GH5 gene and e) either (i) a GH9 gene, (ii) a GH9 gene and a GH12 gene, or (iii) a GH12 gene is disclosed.

Method of creating industrial streptomyces with capability to grow on cellulosic polysaccharide substrates

A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount ofsp. ActE secretome such that at least partial lignocellulosic digestion occurs.

Method and compositions for improved lignocellulosic material hydrolysis

A multifunctional polypeptide capable of hydrolyzing cellulosic materials, xylan, and mannan is disclosed. The polypeptide includes the catalytic core (cc) ofCthe_0797 (CelE), the cellulose-specific carbohydrate-binding module CBM3 of the cellulosome anchoring protein cohesion region (CipA) of(CBM3a), and a linker region interposed between the catalytic core and the cellulose-specific carbohydrate binding module. Methods of using the multifunctional polypeptide are also disclosed.

Multifunctional cellulase and hemicellulase

Methods for the cell-free identification of polypeptide and polypeptide combinations with utility in biomass transformation, as well as specific novel polypeptides and cell-free systems containing polypeptide combinations discovered by such methods are disclosed.

Combinatorial discovery of enzymes with utility in biomass transformation

Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in. At the C terminus and the penultimate position, substitution to residues with charged side chains, large non-polar side chains, or no side chains can be used to reduce or inhibit the protein degradation. At the antepenultimate position from the C terminus, residues with no side chain or acidic side chains can increase the stability, i.e. reduce or inhibit the protein degradation. The combinational substitution of only the last three residues of polypeptides can make the polypeptides more stable during heterologous expression in mycobacterial hosts.

Carboxy terminal residues as predictors and regulators of protein stability

Expression systems and methods for the expression of functional membrane polypeptides such as human cytochrome b5 are provided. The systems include recombinant expression vectors capable of expressing soluble fusion proteins that include a solubilizing agent, a linker, and a membrane polypeptide, as well as one or more cleavers, e.g. proteases, capable of cleaving the linker to release the membrane polypeptide. When the fusion protein is expressed, the linker is cleaved by the cleaver to allow association of the membrane polypeptide with a membrane.

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Madison, WI, United States of America

Brian Fox