Life Technologies Corporation

Brian Filanoski

Kyle R Gee

Aleksey Rukavishnikov

Iain Johnson

Lawrence Greenfield

Schuyler Corry

William Downey

James Hirsch

The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a &#x3b2;-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a &#x3b2;-lactamase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a &#x3b2;-lactamase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a &#x3b2;-lactamase or instructions for using the components in an assay.

Optically-detectable enzyme substrates and their method of use

The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a &#x3b2;-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a &#x3b2;-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a &#x3b2;-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a &#x3b2;-lactmase or instructions for using the components in an assay.

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Eugene, OR, United States of America

Brian Filanoski