Momenta Pharmaceuticals, Inc.

Brian Edward Collins

Carlos J Bosques

Dorota A Bulik

Nathaniel J Washburn

James Meador, Iii

Ian Christopher Parsons

John Robblee

Jay Duffner

Lakshmanan Thiruneelakantapillai

Ganesh Kaundinya

Naveen Bhatnagar

Enrique Arevalo

Kevin Millea

Maurice Gaston Hains

Sean Smith

Zachary Shriver

Ganesh Venkataraman

James R Myette

Holly Prentice

Brett Belongia

Xiangping Zhu

Rajeev Chillakuru

Rasheed Tijani

Jennifer Murphy

Joseph Glajch

Xiaomei He

Yan Yin

Yin Yin Lin

Zachary Schriver

The present disclosure provides, in part, methods for evaluating, identifying, and/or producing (e.g., manufacturing) a CTLA4-Fc fusion protein, e.g., abatacept. In some instances, methods herein allow highly resolved evaluation of abatacept useful for, inter alia, manufacturing abatacept, characterizing abatacept, identifying and/or confirming abatacept, monitoring the structure of abatacept, comparing abatacept preparations made over time or made under different conditions, and/or controlling the structure of abatacept.

Methods related to CTLA4-Fc fusion proteins

Sulfated glycoproteins, and methods of making and using such sulfated glycoproteins, are described.

Modified glycoproteins

The present invention relates to the characterization and production of biologics.

Methods related to biologics

Methods and compositions related to high mannose glycans are described.

High mannose glycans

The present invention provides methods of evaluating a glycoprotein preparation for the absence, presence or amount of an N-acetylhexosamine glycan, e.g., an N-acetylglucosamine glycan.

N-acetylhexosamine-containing N-glycans in glycoprotein products

The present invention relates to the characterization and production of denosumab.

Methods related to denosumab

Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using ammonium and/or lysine, are described.

Methods of cell culture

The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially.

Characterization of N-glycans using exoglycosidases

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The present disclosure provides methods for assessing the glycosylation of a target glycoprotein produced by a cell through analysis of cell-surface glycans on the cell. The present disclosure therefore teaches that glycosylation of cell surface proteins can serve as a proxy for glycosylation of other proteins.

Methods related to cell surface glycosylation

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Arlington, MA, United States of America

Brian Edward Collins