University of Washington

Matchstick Technologies, Inc.

Brian E MacConaghy

Thomas J Matula

Adam D Maxwell

Karol Bomsztyk

Oleg A Sapozhnikov

Jarred Egan Swalwell

Greg P Darlington

Andrew A Brayman

Camilo Perez

Samuel R Browd

Pierre D Mourad

Justin Reed

Revathi Murthy

Nathaniel Coulson

A system for processing biological or other samples includes an array of transducer elements that are positioned to align with sample wells in a microplate. Each transducer element produces ultrasound energy that is focused towards a well of the microplate with sufficient acoustic pressure to cause inertial cavitation. In one embodiment, the transducers are configured to direct ultrasound energy into cylindrical wells. In other embodiments, the transducer elements are configured to direct ultrasound energy into non-cylindrical wells of a microplate.

Ultrasound system for shearing cellular material

Disclosed embodiments include illustrative piezoelectric element array assemblies, methods of fabricating a piezoelectric element array assembly, and systems and methods for shearing cellular material. Given by way of non-limiting example, an illustrative piezoelectric element array assembly includes at least one piezoelectric element configured to produce ultrasound energy responsive to amplified driving pulses. A lens layer is bonded to the at least one piezoelectric element. The lens layer has a plurality of lenses formed therein that are configured to focus ultrasound energy created by single ones of the at least one piezoelectric element into a plurality of wells of a microplate disposable in ultrasonic communication with the lens layer, wherein more than one of the plurality of lenses overlie single ones of the at least one piezoelectric element.

Ultrasound system for shearing cellular material in a microplate

Embodiments are generally related to differentiating and/or separating portions of a sample that are of interest from the remainder of the sample. Embodiments may be directed towards separating cells of interest from a cell sample. In some embodiments, acoustic impedances of the cells of interest may be modified. For example, the acoustic properties of the cells of interest may be modified by attaching bubbles to the cells of interest. The cell sample may then be subjected to an acoustic wave. The cells of interest may be differentiated and/or separated from the remainder of the sample based on relative displacements and/or volumetric changes experienced by the cells of interest in response thereto. The cells of interest may be separated using a standing wave and sorted into separate channels of a flow cell. Optionally, the cells may be interrogated by a light source and differentiated by signals generated in response thereto.

Methods for separating, concentrating, and/or differentiating between cells from a cell sample

A stylet () includes a handle assembly () with an indicator display () and a stiff wire assembly () extending distally from the handle assembly () having a non-imaging ultrasonic device on a distal end. The stylet includes a circuit assembly having one or more of a pulser (), a transmit/receive chip (), a bandpass filter (), a differential amplifier (), an ADC (), and an MCU (), operable to control the operation of the ultrasonic device and to receive and analyze data from the ultrasonic device to facilitate implantation of a device such as a catheter.

Ultrasound stylet

Systems, devices, and methods for separating, concentrating, and/or differentiating between cells from a cell sample

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Seattle, WA, United States of America

Brian E MacConaghy