Illumina Corporation

Illumina Cambridge Limited

E.i. Dupont De Nemours and Company

Hewlett-packard Development Company, L.p.

Illumina Singapore Pte. Ltd.

Brian Douglas Mather

Wayne N George

Maria Candelaria Rogert Bacigalupo

Lewis J Kraft

Boyan Boyanov

Andrew Antony Brown

Jeffrey S Fisher

Ludovic Vincent

M Shane Bowen

Sang Ryul Park

Justin Fullerton

Sahngki Hong

Xavier Von Hatten

Susan Marie Hennessey

Gianluca Andrea Artioli

Dean M Fake

Tyler D Pritchett

Hayden Black

Maxwell Zimmerley

Jennifer S Ovental

Wayne Atkinson

Negash Adugna

Tarun Kumar Khurana

Xiaohai Liu

Jeffrey G Mandell

Hai Quang Tran

Yir-Shyuan Wu

Michael Stephen Wolfe

Dajun Yuan

John Donald Summers

Cyril Delattre

Alexandre Richez

Randall Smith

Antoine Francais

Behnam Javanmardi

Sean M Ramirez

Xiaohe Chen

John M Gardner

Hee Hyun Lee

Bryan Benedict Sauer

Mathieu Lessard-Viger

Hong Xie

Hoang Vi Tran

Filiz Gorpe-Yasar

Julia Morrison

Yuefei Tao

Steven Modiano

Daniel Leonard Fuller

Esther Kim

Wei Wei

Edwin Li

Kaitlin Pugliese

Yan Zhu

Raymond Campos

Krystal Sly

Innsu Daniel Kim

Sara Bakhshi

Pietro Gatti Lafranconi

Ma Helen Cativo

Sebastien Georg Gabriel Ricoult

Sojeong Moon

Ryan Sanford

Pallavi Daggumati

John Morrow Gardner

Silke Musa

Sang Park

Weixian Xi

An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3' OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.

Kits and flow cells

In one example, a method of preparing a fluidic channel includes covalently coupling a first region of a substrate to a first region of a cover using first moieties covalently coupled to the first region of the substrate and second moieties covalently coupled to the first region of the cover. The covalent coupling between the first region of the substrate and the first region of the cover suspends a second region of the cover over a second region of the substrate to form a fluidic channel.

Fluidic devices including fluidic channels, and methods of making the same

An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.

Functionalized plasmonic nanostructures

In an example, a sensing system includes a pH sensor. The pH sensor includes two electrodes and a conductive channel operatively connected to the two electrodes. A complex is attached to the conductive channel of the pH sensor. The complex includes a polymerase linked to at least one pH altering moiety that is to participate in generating a pH change within proximity of the conductive channel from consumption of a secondary substrate in a fluid that is exposed to the pH sensor. The at least one pH altering moiety is selected from the group consisting of an enzyme, a metal coordination complex, a co-factor, and an activator.

Sensing systems

An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

Flow cells

In one example, a flow cell includes a substrate, an electrode positioned on the substrate, and a patterned material positioned on the electrode. In this example, the patterned material includes depressions separated by interstitial regions, and a functionalized surface of the electrode is exposed at each of the depressions. In this example, the flow cell further includes a primer grafted to the functionalized surface in each of the depressions. In another example, a flow cell includes a substrate and a patterned electrode positioned on the substrate. In this other example, the patterned electrode includes depressions separated by interstitial regions, and a functionalized surface of the substrate exposed at each of the depressions. In this other example, a primer is grafted to the functionalized surface in each of the depressions.

Flow cells and sequencing kits

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

In the examples set forth herein, nucleic acid extraction materials are capable of selectively extracting cell free nucleic acids, including cell free DNA, directly from whole blood samples or plasma. Also included are methods of making and using the nucleic acid extraction materials. One example of a nucleic acid extraction material includes a substrate. This example of the nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. In this example, the polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.

Nucleic acid extraction materials, systems, and methods

A conductive paste composition comprises (i) an inorganic powder comprising at least a conductive powder, (ii) at least one microgel polymer, and (iii) a solvent. The paste composition may be used in a process for manufacturing an electrical device comprising: preparing a substrate; applying the conductive paste onto the substrate in a preselected pattern; and heating the applied conductive paste to form a conductive structure that provides an electrode for connecting the device. The paste composition beneficially permits the formation of narrow, high aspect ratio features in the conductive structure.

Conductive paste composition and semiconductor devices made therewith

Reaction solutions are disclosed herein comprising water, incompletely refined sucrose, and a glucosyltransferase enzyme that synthesizes insoluble poly alpha-1,3-glucan having at least 50% alpha-1,3 glycosidic linkages and a weight average degree of polymerization (DP) of at least 100. The yield of poly alpha-1,3-glucan by a reaction solution herein is at least 7% of the weight of sucrose that was converted in the reaction solution. Further disclosed are methods of producing poly alpha-1,3-glucan using incompletely refined sucrose, and poly alpha-1,3-glucan produced by these methods.

Production of glucan polymers from alternate sucrose sources

The present disclosure is drawn to an ink-jet ink and a method of ink-jet printing over a prolonged period of time. The ink-jet ink include from 0.1 wt % to 10 wt % pigment by solids, from 0.1 wt % to 15 wt % latex by solids, and from 0.01 wt % to 3 wt % phosphate-containing surfactant, wherein when the ink-jet ink is fired from a thermal ink-jet printhead at 400 million drops per nozzle with said nozzle having an orifice size of about 20 microns, at least 80% drop weight is retained compared to an initial firing prior to firing the 400 million drops per nozzle.

Phosphate-containing surfactants for use with pigment&#x2014;and latex-containing ink-jet inks

Growing community of inventors

San Diego, CA, United States of America

Brian Douglas Mather