Purdue Research Foundation

Brian Dilkes

Gurmukh S Johal

Mitchell R Tuinstra

Elizabeth Buescher

Kartikeya Krothapalli

Presented herein are methods for creating a desired genetic variant at a high efficiency from a seed. In one aspect, a method for creating a desired genetic variant at a high efficiency from a seed is presented. The method involves subjecting isolated pollen to a chemical mutagen to produce a first generation seed, planting the first generation seed in (n) plots to produce first generation plants, recovering mutagenized pollen from the first generation plants, pollinating a female parent with the recovered mutagenized pollen to produce a next generation seed, subjecting pollen from the next generation seed to mutagenesis to pollinate the first generation plant, resulting in a new next generation seed, planting the new next generation seed to produce a new next generation plant, and subjecting pollen from the new next generation plant to mutagenesis to pollinate the new next generation plant.

Induced mutagenesis

Embodiments of the present invention relate generally to new forage crops and methods of creating new forage crops. For example, chemical mutagenesis is used to create mutant crops having desirable forage characteristics. In some embodiments, a mutantplant is produced by inducing mutagenesis; producing a population of mutantplants using the treated reproductive portion; assaying the population of mutantfor a C493Y mutation in CYP79A1; and growing the mutantplant, wherein the mutantplant exhibits altered dhurrin content or catabolism as compared to a controlplant.

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Lafayette, IN, United States of America

Brian Dilkes