Pierce Biotechnology, Inc.

Brian David Wolf

Surbhi Desai

Peter T Czerney

Matthias S Wenzel

Frank G Lehmann

Brian Webb

Bernd G Schweder

Gregory John Kilmer

Gary G Judd

Scott Eugene Starwalt

Zakir S Murtaza

A semi-dry, one step electroblot transfer buffer composition for rapid transfer of proteins or polypeptides from polyacrylamide gel to a suitable membrane such as nitrocellulose or polyvinylidene difluoride (PVDF). The composition contains components that minimized electrical resistance and enabled high efficiency rapid semi-dry transfer using conventional readily available filter paper, i.e., cotton cellulose fiber.

Electroblot transfer buffer

A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.

Protein detection and quantitation using hydroxyquinolone dyes

Coumarine/chinolone and Martina-type compounds, compositions, and method of using the compounds and compositions for protein detection, assay, quantitation, etc. are disclosed. The composition, also referred to as a dye, has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc.

Protein probe compounds, compositions, and methods

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Machesney Park, IL, United States of America

Brian David Wolf