Ventana Medical Systems, Inc.

Ventana Medic Systems, Inc.

The New Guilds, LLC

Nihon University School of Medicine

Brian Daniel Kelly

Christopher Bieniarz

Nathan Walter Polaske

Julia Ashworth-Sharpe

Mark Lefever

Jerome W Kosmeder, Ii

Eric J May

Phillip C Miller

Adrian E Murillo

Rui Hong

Jan Froehlich

Hong Wang

Larry E Morrison

Michael Patrick Farrell

Yuri Belosludtsev

Junshan Hao

Traci D DeGeer

Wendy J French

Donald D Johnson

Noemi Sebastiao

Wenjun Zhang

Taylor J Shingler

Lei Tang

Thomas M Grogan

Shinobu Masuda

Stacey Stanislaw

Nelson Alexander

Fabien Gaire

Alisa Tubbs

Antony Hubbard

James Grille

Pamela Wirth

Chol Steven Yun

Hiro Nitta

Pascal Bamford

Hiroaki Nitta

Megan Lehrkamp

Mary Padilla

Ted Williams

Richard Gniewek

Eslie Dennis

Leigh A Henricksen

Jerome Kosmeder

Donald Johnson

Applicants have developed an amplification system and methodology for IHC and ISH staining that utilizes 'click chemistry' to covalently bind reporter molecules to tissue.

Application of click chemistry for signal amplification in IHC and ISH assays

Disclosed herein are novel chromogenic conjugates, the conjugates comprising at least two detectable moieties.

Colors for chromogenic IHC and ISH staining with multi-dye quinone methide and tyramide conjugates

Disclosed herein are novel quinone methide analog precursors and embodiments of a method and a kit of using the same for detecting one or more targets in a biological sample. The method of detection comprises contacting the sample with a detection probe, then contacting the sample with a labeling conjugate that comprises an enzyme. The enzyme interacts with a quinone methide analog precursor comprising a detectable label, forming a reactive quinone methide analog, which binds to the biological sample proximally to or directly on the target. The detectable label is then detected. In some embodiments, multiple targets can be detected by multiple quinone methide analog precursors interacting with different enzymes without the need for an enzyme deactivation step.

Quinone methide analog signal amplification

Disclosed herein are caged haptens and caged hapten-antibody conjugates useful for enabling the detection of targets located proximally to each other in a sample.

Protein proximity assay in formalin fixed paffafin embedded tissue using caged haptens

Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

Polymer stabilization of chromogen solutions

Particular disclosed embodiments disclosed herein concern using a one or more various mass tags, which can be specifically deposited at targets through direct or indirect enzymatic-catalyzed transformation, to provide a method for identifying targets in tissue samples. The mass tags may be labeled with stable isotopes to produce mass tags having the same chemical structure but different masses. Mass codes produced by ionizing the mass tags are detected and/or quantified using mass spectrometry. The method can be used for multiplexed detection of multiple targets in a particular sample. In some embodiments, a map divided into sections representing sections of the tissue sample may be prepared, with the map sections including data corresponding to quantification data wherein the size of a mass peak is determined and correlated with the amount of a target for the corresponding tissue sample section.

Detecting targets using mass tags and mass spectrometry

Disclosed are compositions and methods for inactivating one or more enzymes in a biological sample.

Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays

Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.

Single-stranded oligonucleotide probes for chromosome or gene copy enumeration

Protein proximity assay in formalin fixed paraffin embedded tissue using caged haptens

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Tucson, AZ, United States of America

Brian Daniel Kelly