Tm Technologies, Inc.

The State University of New York

Other

Luminex Molecular Diagnostics, Inc.

Brian D Faldasz

Michael J Lane

Albert S Benight

Jerrie Gavalchin

The invention provides reagents and methods for lateral flow assays and quantitative capture or determination of components, including cells, in a sample. In one aspect, reagents and methods for diagnostic assay are provided. In one embodiment an assay for determining T cell numbers, particularly a CD2+ CD4+ T cell assay is provided. A manufacturing method for producing rapid diagnostic assays in a decentralized manner is also described. The method generates net economic advantages over conventional diagnostic manufacturing practices.

Lateral flow based methods and assays for rapid and inexpensive diagnostic tests

The invention relates to the field of molecular biology, nucleic acid chemistry and medical diagnostics. More specifically, it relates to methods and compositions for promoting the hybridization of a nucleic acid probe with a target nucleic acid sequence which is not perfectly matched to the probe.

Methods and compositions for modulating 'marginally indiscriminant' hybridizations

Methods for detecting the presence or absence of an analyte in a sample are disclosed. Kits for performing the analysis methods of the invention are also disclosed.

Signal amplification method

Methods, compositions, and kits for modulating the stability of at least one nucleic acid duplex, are disclosed.

Methods and compositions for modulating melting temperatures of nucleic acids

Methods for identifying a site on a nucleic acid sequence having a desired free energy variability are disclosed. The methods are useful for, e.g., selecting antisense oligonucleotides, e.g., for therapeutic use.

Process for selecting anti-sense oligonucleotides

A method of providing the sequence of a single stranded nucleic acid molecule, which, when hybridized to a complementary single stranded molecule, results in a double stranded (duplex) structure having a preselected value for a free energy parameter.

Thermodynamics, design, and use of nucleic acid sequences

Nucleic acid capture moieties, methods of using nucleic acid capture moieties, reaction mixtures including nucleic acid capture moieties, and kits including nucleic acid capture moieties are disclosed.

Nucleic acid capture moieties

A method for determining at least one DNA sequence that can be employed as a flanking sequence to a DNA binding site for a ligand, wherein the DNA binding site is located within a preselected DNA sequence, to provide a relative increase, decrease, or approximate equality in binding constant of the ligand for its DNA binding site, or relative composite reaction rate for the ligand and a DNA sequence. Also provided are applications of the method to determine DNA primers useful in conjunction with DNA amplification to detect the presence or absence of DNA sequences and to diagnose diseases related to viral DNA, such as human immunodeficiency virus.

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Maynard, MA, United States of America

Brian D Faldasz