454 Life Sciences Corporation

Brian Christopher Godwin

John Harris Leamon

Michael Egholm

Jan Berka

Gary James Sarkis

Jan Fredrik Simons

Zhoutao Chen

Gianni Calogero Ferreri

Stephen Kyle Hutchison

Priya Shanbhag

Melinda Palmer

Craig Elder Mealmaker

Shally Hsueh-Wen Wang

An embodiment of a method for generating a population of amplified concatamer products is described that comprises amplifying a template nucleic acid molecule using a first nucleic acid primer immobilized on a bead substrate and a second nucleic acid primer in solution to generate a population of substantially identical copies of the template nucleic acid molecule immobilized on the bead substrate; and amplifying the population of substantially identical copies of the template nucleic acid molecule using a concatamer primer that comprises a first region complementary to an end region of the population of substantially identical copies of the template nucleic acid molecule and a second region to generate a population of immobilized concatamer products of the substantially identical copies of the template nucleic acid molecule.

System and method for nucleic acid amplification

The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.

Paired end sequencing

Growing community of inventors

North Haven, CT, United States of America

Brian Christopher Godwin