Roche Sequencing Solutions, Inc.

Kapa Biosystems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Brian Christopher Godwin

Joseph Platzer

Sedide Ozturk

Jan Berka

Carolina Dallett

Seoyoung Kim

Bronwen Miller

Jo-Anne Elizabeth Penkler

Janine Mok

Khai Luong

Florian Rubelt

Daniel Burgess

Richard Dannebaum

Alexander Lovejoy

Jainee Lewis

Martin Ranik

Dilduz Telman

Priya Shanbhag

Melinda D Palmer

Jo-Anne Penkler

Gianni Calogero Ferreri

Craig Elder Mealmaker

Shally Hsueh-Wen Wang

Improved methods and compositions are provided herein for primer extension target enrichment of target polynucleotides.

Primer extension target enrichment

The present invention is a method and compositions for primer extension target enrichment of nucleic acids and improvements thereto including simultaneously enriching for RNA and DNA and optionally sequencing the enriched products.

Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA

The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. A first oligonucleotide is hybridized to a target nucleic acid in library of nucleic acids having first and second adapters. The hybridized first oligonucleotide is extended with a first polymerase, thereby producing a first primer extension complex including the target nucleic acid and the extended first oligonucleotide. The first primer extension complex is captured, enriched relative to the library of nucleic acids, and a second oligonucleotide is hybridized to the target nucleic acid. The hybridized second oligonucleotide is extended with a second polymerase, thereby producing a second primer extension complex including the target nucleic acid and the extended second oligonucleotide, and further liberating the extended first oligonucleotide from the first primer extension complex.

Target enrichment by unidirectional dual probe primer extension

Methods and compositions are described herein for primer extension target enrichment of immune receptor (BCR or TCR) sequences.

Immune profiling by primer extension target enrichment

The invention comprises a method of amplifying nucleic acids by primer extension with reduced formation of primer-primer byproducts.

Elimination of primer-primer interactions during primer extension

The present invention relates to a kit and a method of linear amplification of a least one nucleic acid target in a sample, said method comprising: (a) contacting each target in the sample with a nucleic acid polymerase and a primer comprising a component preventing copying of the primer by the nucleic acid polymerase; and at least one nuclease blocking nucleotide; (b) generating a primer extension product; (c) preventing priming by the 3'-end of the primer extension product, and (d) repeating steps b) and c) at least once.

Methods to amplify highly uniform and less error prone nucleic acid libraries

Immuno-PETE

The present invention is a method and compositions for primer extension target enrichment of nucleic acids and improvements thereto including simultaneously enriching for RNA and DNA and optionally sequencing the enriched products. An embodiment of the present invention includes a method comprising the steps of: hybridizing a target-specific primer to a target DNA or RNA, wherein the primer comprises a target-binding region and a region of complementarity to an adaptor; extending the primer with a DNA polymerase or reverse transcriptase to form a primer extension product; contacting the product with an adaptor comprising a longer strand with a 5'-overhang having complementarity to said primer and a shorter strand comprising a universal priming site; hybridizing the adaptor to the product; and ligating one strand of the adaptor to the product to form a ligation product.

The invention comprises methods and compositions for enriching for a target nucleic acid with a single primer extension and low-bias limited amplification.

Target enrichment by single probe primer extension

Growing community of inventors

Livermore, CA, United States of America

Brian Christopher Godwin