Van Andel Research Institute

University of Pittsburgh

Detroit R & D, Inc.

Brian Brummeler Haab

Randall E Brand

Hyesook Kim

David M Kruithoff

Alan A Dombkowski

Raymond F Novak

Songming Chen

Bryan Reatini

Tingting Yue

A method of diagnosing pancreatic cancer in a patient by detecting a level of one or more glycoforms of a Lewis antigen and a level of the one or more glycoforms of MUC5AC. The patient diagnosed with pancreatic cancer then may be treated for this disease. Also, a method for detecting a level of a glycan in a sample which includes using a capture reagent to immobilize the glycan on a substrate; exposing the immobilized glycan to a detection reagent; detecting the level of the immobilized glycan; and combining the biological sample with one or more pre-capture enzymes and/or exposing the immobilized glycan to one or more pre-detection enzymes.

Methods for detecting and for treating pancreatic cancer

Method of detecting the level of a glycan

An antibody microarray screen including a substrate, monoclonal and polyclonal antibodies that are purified immunoglobins, wherein the antibodies are spotted on predetermined positions on the substrate, and fluids unprocessed for immunoglobulin isolation (e.g., anti-sera, ascites fluids, or hybridoma culture media), wherein the unprocessed fluids are spotted on the predetermined positions on the substrate. Production of drug-metabolizing enzyme antibody microarrays containing closely related cytochromes P450 is disclosed. Methods of manufacturing an antibody microarray, an internal control molecule for use in an antibody microarray, a method of determining optimal spotting concentrations of IgG and a method to increase a detectable signal with microarray analysis are disclosed.

Chip production, hybridization and data interpretation for antibody and protein microarrays

Methods and related kits for differentiating pancreatic cancer from a benign pancreatic disease. The method includes assaying a patient biological sample for a total level of CA 19-9 antigen and for a glycan level in specific mucin(s), and comparing the total level of CA 19-9 antigen and the glycan level in the specific mucin(s) to statistically validated thresholds, wherein a different level of total CA 19-9 antigen in the patient biological sample as compared to a statistically validated threshold and a different level of glycan level in the specific mucin(s) as compared to statistically validated thresholds indicate pancreatic cancer in the patient rather than a benign pancreatic disease.

Biomarkers for pancreatic cancer and diagnostic methods

The invention includes a high-through-put method of screening for a biomarker indicative of a disease, including the identification of a glycan alteration in a protein of a diseased patient. Further, the invention includes methods utilizing a microarray to measure the glycan level of a protein.

Methods for measuring glycan levels of proteins

An apparatus for placing a hydrophobic barrier on an examination slide including a vessel, a printer block having a printing surface to form a hydrophobic barrier on the examination slide in a specified pattern, a slide holder, and a reversible actuator. The invention also includes a method for placing a hydrophobic barrier on an examination slide in a specified pattern.

Growing community of inventors

Jenison, MI, United States of America

Brian Brummeler Haab