Harvard College

Brian Beliveau

Chao-ting Wu

Yu Albert Wang

Peng Yin

Jocelyn Yoshiko Kishi

Sinem K Saka

Yu Wang

Provided herein, in some embodiments, are methods and compositions for highly multiplexed in situ signal amplification via hairpin-mediated concatemerization.

Multiplexed signal amplification

Novel methods and compositions for identifying one or more factors associated with a nucleic acid sequence (e.g., DNA and/or RNA) of interest are provided.

Oligonucleotide trapping

The present invention relates to methods of making linear nucleic acid probes using rolling circle amplification methods.

Methods of making oligonucleotide probes

The present invention relates to methods of hybridizing nucleic acid probes to genomic DNA.

Methods of identifying homologous genes using FISH

The present invention relates to methods of making linear nucleic acid probes using rolling circle amplification methods. In one aspect, an oligonucleotide template sequence is contacrted with one or more first primers, a first polymerase and first nucleotides under conditions that extend one or more hybridize primers to make a strand complementary to the oligonucleotide template sequence thereby forming a double stranded oligonucleotide. The double stranded oligonucleotide is circularized. A rolling circle template is obtained from the circularized double stranded oligonucleotide. The rolling circle template is contacted with one or more second primers, a second polymerase and second nucleotides under conditions that form a single stranded nucleic acid, and the single stranded nucleic acid is cleaved to form the plurality of nucleic acid probes.

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Cambridge, MA, United States of America

Brian Beliveau