Canon U.S. Life Sciences, Inc.

Other

Canon U.s.a., Inc.

Qiagen Gaithersburg Inc.

Separation Technology, Inc.

Brian Bean

Kenton C Hasson

Johnathan Stuart Coursey

Scott Corey

Brian P Murphy

Ben Lane

Gregory H Owen

Hongye Liang

Sami Kanderian

Eric D Schneider

Brian Lee Lipford

Shulin Zeng

Hiroshi Inoue

Alex K Flamm

Ivor T Knight

Weidong Cao

Conrad Laskowski

Takayoshi Hanagata

Ying-Xin Wang

Franklin Regan

Chris Dykes

Bradley Scott Thomas

Samuel Reed

Eva J Dixon

Steve Marlow

Mike Abbott

Eva Dixon

Brian Murphy

Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.

Methods, devices, and systems for processing multiple assays

The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

System and method for serial processing of multiple nucleic acid assays

Systems for processing multiple assays background

The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.

Slug control during thermal cycling

A method for visually inspecting pelletized samples. The method generates an inspection image of the bottom of a sample tube holder. The sample tube holder has a number of sample tube locations, and each sample tube location is configured to receive a sample tube in it. The inspection image is evaluated to determine whether a tube image exists for each sample tube location, and each sample tube image is evaluated to determine whether a pellet is located in each sample tube.

Automated pelletized sample vision inspection apparatus and methods

A fluid sampling device () that operates by a combination of capillary action to collect a small fluid sample, and by pressure differential when inserted into an analyzer () to expose the fluid sample for testing by the analyzer (). The device is especially suited for use as a disposable blood sampling unit designed to interface with a blood analyzer, albeit the concept of the invention may be employed for sampling and testing virtually any fluids.

Disposable fluid sample collection device

Ultrasonic determination of red blood cell indices device

Growing community of inventors

Baltimore, MD, United States of America

Brian Bean