Arizona State University

Brian Ashcroft

Deirdre Meldrum

Laimonas Kelbauskas

Roger H Johnson

Jeff Houkal

Hong Wang

Jakrey Myers

Rishabh Shetty

Iniyan Soundappa Elango

Dean Smith

Shih-Hui Joseph Chao

Shih-Hui (Joseph) Chao

Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-involves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.

Live-cell computed tomography

Systems and methods of using the same for functional fluorescence imaging of live cells in suspension with isotropic three dimensional (3D) diffraction-limited spatial resolution are disclosed. The method-live cell computed tomography (LCCT)-in-volves the acquisition of a series of two dimensional (2D) pseudo-projection images from different perspectives of the cell that rotates around an axis that is perpendicular to the optical axis of the imaging system. The volumetric image of the cell is then tomographically reconstructed.

Growing community of inventors

Mesa, AZ, United States of America

Brian Ashcroft