Exact Sciences Development Company, LLC

Exact Sciences Corporation

Third Wave Technologies, Inc.

Renovar, Inc.

Brian Aizenstein

Hatim T Allawi

Graham P Lidgard

Michael J Domanico

Oliver Hunt

Melissa M Gray

Tobias Charles Zutz

Michael W Kaiser

Abram Michael Vaccaro

Tamara J Sander

Maria Giakoumopoulos

Victor I Lyamichev

James Robert Prudent

Jeff G Hall

Mary Ann Brow

David J Marshall

Huaizhong Hu

Andrea L Mast

Poonam Agarwal

Scott M Law

Stuart Knechtle

Ilse A Tyler

Alice Puchalski

Jennifer M Schneiders

Mary C Oldenberg

Carolyn W Miller

Monika De Arruda Indig

Deborah E Burris

Myrta W Atiles

David Arco

Eric B Rasmussen

The present invention provides synthetic DNA strands that find use as process controls in DNA processing and nucleic acid testing methods. In particular, provided herein are synthetic methylated DNA strands of known composition for use as control molecules in DNA testing, e.g., of mutations and/or methylation of DNA isolated from non-fish samples, such as human samples.

Nucleic acid control molecules from non-human organisms

Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.

Modification of DNA on magnetic beads

Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

Isolation and detection of DNA from plasma

The present invention provides DNA plasmids that find use as calibrators or reference standards for calculating DNA quantities in a sample. In particular, provided herein are DNA plasmids that contain multiple control fragments, and methods of their use in DNA quantitation.

Multiple-control calibrators for DNA quantitation

Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfate conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads.

The present invention relates to methods of diagnosing, predicting and monitoring kidney disorders. In particular, the present invention relates to the diagnosis, prediction and monitoring of kidney disorders by detection of cytokines, cytokine-related compounds and chemokines in urine. The present invention further relates to methods and compositions for assessing the efficacy of agents and interventions used to treat kidney disorders.

Systems and methods for characterizing kidney diseases

The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5' nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

Methods and compositions for detecting target sequences

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Madison, WI, United States of America

Brian Aizenstein