Other

Pioneer Hi-bred International, Inc.

Purdue Research Foundation

Lubrizol Genetics, Inc.

The University of Arizona

Arizona Board of Regents, a Body Corporate

Brian A Larkins

William James Gordon-Kamm

Keith S Lowe

Brian R Dilkes

George J Hoerster

Carolyn A Gregory

Ronald D Burnett

Matthew A Bailey

Young Min Woo

Richard E Cuellar

Yuejin Sun

Gary Allen Thompson

Gad Galili

John C Wallace

Wallace John C

Ricardo A Dante

Jeffrey E Habben

Gloverson L Moro

The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for increasing transformation frequencies, increasing crop yield, providing a positive growth advantage, modulating cell division, transiently modulating cell division, and for providing a means of positive selection.

Methods of using viral replicase polynucleotides and polypeptides

The invention provides isolated nucleic acids and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content, cell cycle progression, cell number and/or composition of plants.

Cell cycle nucleic acids, polypeptides and uses thereof

The invention provides isolated nucleic acids and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content, cell cycle progression and/or composition of plants.

The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for modulating endoreduplication and increasing crop yield.

Seed lysine is detected by an immunoassay in which anti-EF antibody binds seed protein. Seed EF concentration is highly correlated with seed lysine content.

Method for estimating the lysine content of seed by elongation factor

A gene construct comprising SEQ ID NO:3, a phloem-specific promoter, and protein encoding nucleotide sequence not natively associated with SEQ ID NO:3 is disclosed. A gene construct comprising a protein encoding nucleotide sequence not natively associated with SEQ ID NO:3 and a sufficient portion of SEQ ID NO:3 such that the portion promotes the preferential expression of the protein-encoding nucleotide sequence in phloem tissue is also disclosed. A gene construct comprising a promoter sequence from a PP2-type gene isolated from a Cucurbita and a protein encoding sequence not natively associated with the promoter is disclosed.

Phloem-specific promoter

Modified 19kd and 22kd zeins containing lysine are provided. Lysine additions are made by modifying structural genes which encode 19kd and 22kd pre-zeins. Modified zeins produced from the modified structural gene templates are found to retain important properties of unmodified zeins, having similar molecular weights, having the ability to form protein bodies within the rough endoplasmic reticulum of a host cell and being soluble in alcohol.

Modified zein genes containing lysine

Modified zein proteins containing lysine

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Tucson, AZ, United States of America

Brian A Larkins