Agilent Technologies, Inc.

Brian A Bidlingmeyer

Klaus E Witt

Edwin E Wikfors

Alan D Broske

Qunjie Wang

A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit; pressurizing the sample in the sample loop with the pressurized first solvent, while the mobile phase continues to be directed directly into the separation unit; and switching the sample loop into the mobile phase, thereby introducing the pressurized sample to the separation unit.

Apparatus and method for introducing sample into a separation unit of a chromatography system without disrupting a mobile phase

The present invention provides silica-based reversed-phase HPLC methods that lead to higher retention of the analytes in the column and longer column lifetimes than usually observed under medium to high pH aqueous mobile phase conditions. The inventive methods comprise eluting the HPLC column using an aqueous mobile phase comprising at least one fluorinated additive. Preferred additives are polyfluorinated alcohols such as 2,2,2-trifluoroethanol and 1,1,1,3,3,3-hexafluoroisopropanol. The methods of the present invention may be used for analyzing, separating, purifying, and/or isolating small organic molecules, natural products, as well as biomolecules such as polypeptides, oligonucleotides and polynucleotides (e.g., DNA fragments).

Additives for reversed-phase HPLC mobile phases

The subject invention includes methods and devices for separating at least two constituents of a highly aqueous fluid. The subject methods include contacting a highly aqueous fluid with a stationary phase having a low density hydrophobic bonded phase under conditions to separate at least two constituents. Also provided are systems that include a stationary phase having a low density hydrophobic bonded phase, a highly aqueous fluid comprising at least two constituents and a device configured to perform liquid chromatography. Kits for use in practicing the subject methods are also provided.

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Frazer, PA, United States of America

Brian A Bidlingmeyer