Grifols, S.a.

Grifols Therapeutics Inc.

Brett Buno

Joann Hotta

Terri Journigan

Michael Burdick

Danuta Wronska

Douglas C Lee

Todd M Gierman

Chris Glenn

Burton Beams

Stefan Burde

Lori Rinckel

A method of purification of non-enveloped or pseudo-enveloped virus produced in vitro uses a composition with at least one detergent. A method of purification can use multiple detergents, and a method of determining the presence and/or level of a non-enveloped or pseudo-enveloped virus in a sample includes treating with at least one detergent.

Methods for purification of a virus produced in vitro and clearance assay for the virus

Polybrene as an additive in cell culture medium is used in methods for the generation of high-titer hepatitis E virus stocks and assays for titration of hepatitis E virus. A cell culture medium containing polybrene is used for high-titer HEV generation, a method for determining the presence and/or the level of HEV in a sample, and an HEV titration assay using polybrene.

Method for generating high-titer hepatitis E virus stocks and titration assay for hepatitis E virus

Nucleic acid molecules derived from sequences of novel human parvovirus B19 variant genomes are provided. Also provided are assays and kits comprising the nucleic acid molecules.

Human erythrovirus

Methods for detection of parvovirus B19

Growing community of inventors

Durham, NC, United States of America

Brett Buno