Newsouth Innovations Pty Limited

Universitetet I Oslo

Vestlandets Innovasjonsseiskap as

Ewsouth Innovations Pty Limited

Vestlandets Innovasjonsselskap as

Brett A Neilan

Ralf Kellmann

Kjetill Sigurd Jakobsen

Shauna Ann Murray

Russel J S Orr

Janine Naomi Copp

Troco Kaan Mihali

Rabia Mazmouz

Young Jae Jeon

Anke Stuken

Anke Corinna Stüken

Alex Roberts

Anne-Dorothee Jungblut

Tianzhe Liu

Sarah Ongley

Anne Dorothee Jungbult

Russel J Orr

The present invention relates to processes to make neosaxitoxin, and analogues and variants thereof, and intermediates in the production of neosaxitoxin in recombinant host cells. Neosaxitoxin and the analogues and variants thereof may be used in the production of pharmaceutical compositions.

Processes to make neosaxitoxin and analogues thereof

The present invention relates generally to methods for producing microcystin and recombinant cells capable of producing microcystin. The recombinant cells express exogenous microcystin synthase polypeptides under the control of an exogenous promoter, and further express an exogenous phosphopantethienyl transferase (PPTase). The present invention further relates to microcystin as produced in recombinant cells by the methods described herein.

Recombinant microcystin production

The invention generally relates to the field of saxitoxins and the identification of microorganisms capable of producing them. More specifically, the invention relates to the identification of genes encoding saxitoxin in dinoflagellates, and methods for the specific detection of dinoflagellates that are producers of saxitoxins.

Detection of saxitoxin-producing dinoflagellates

Process

The present invention provides methods for the detection of cyanobacteria, and in particular, methods for the detection of cyanotoxic organisms. The invention further relates to methods of screening for compounds that modulate the activity of polynucleotides and/or polypeptides of the saxitoxin biosynthetic pathways.

Cyanobacteria saxitoxin gene cluster and detection of cyanotoxic organisms

The current disclosure generally relates to the field of saxitoxins and the identification of microorganisms capable of producing them. In particular, the saxitoxin A (sxtA) gene comprising catalytic domain sequences saxitoxin A1 (sxtA1) and saxitoxin A4 (sxtA4) is identified in a number of dinoflagellate species. The disclosure relates to methods of detection of saxitoxin-producing dinoflagellates by amplification and detection of the sxtA gene (in particular by PCR) and kits and primers for use in the method are also disclosed. Saxitoxin-producing dinoflagellate genera detected by the method includeor

The invention relates to methods for producing secondary metabolites using transformedsp. bacteria, and secondary metabolites produced by transformedsp. bacteria. The invention further relates to phosphopantetheinyl transferase enzymes.

Methods for producing secondary metabolites

The present invention relates to methods and kits for the detection of toxic cyanobacteria, in particular of hepatotoxin-producing cyanobacteria.

Detection of hepatotoxic cyanobacteria

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Maroubra, Australia

Brett A Neilan