Children's Hospital Research Center at Oakland

Children's Hospital & Research Center Oakland

Childern's Hospital & Research Center at Oakland

Brent T Hagen

Gregory R Moe

The invention relates to methods of producing, and compositions comprising, an isolated alpha (2&#x2192;8) or (2&#x2192;9) oligosialic acid derivative bearing a non-reducing end enriched for one or more de-N-acetyl residues and resistant to degradation by exoneuraminidase. A representative production method involves: (i) treating an alpha (2&#x2192;8) or (2&#x2192;9) oligosialic acid precursor having a reducing end and a non-reducing end with sodium borohydride under conditions for de-N-acetylating the non-reducing end; and (ii) isolating alpha (2&#x2192;8) or (2&#x2192;9) oligosialic acid derivative having one or more de-N-acetylated residues and a non-reducing end that is resistant to degradation by exoneuraminidase. Isolated alpha (2&#x2192;8) or (2&#x2192;9) oligosialic acid derivatives that comprise a non-reducing end de-N-acetyl residue are provided, as well as antibodies specific for the derivatives, compositions comprising the derivatives, kits, and methods of use including protection against and detection ofK1 andbacterial infection, and in diagnosing and treating cancer.

Oligosialic acid derivatives, methods of manufacture, and immunological uses

The invention relates to inhibitors of polysialic (PSA) de-N-acetylase, methods of their production and use. The methods involve use of a PSA de-N-acetylase inhibitor for modifying the growth of cells, such as inhibiting the growth of cancer cells. The compositions include an inhibitor of a PSA de-N-acetylase, such as N-substituted derivatives of the amino sugars hexosamine and neuraminic acid, as well as conjugates and aggregates. Also provided are pharmaceutical compositions that include a PSA de-N-acetylase inhibitor of the invention. Kits containing one or more inhibitor compositions of the invention, as well as methods of preparing the compositions also are provided.

Inhibitors of polysialic acid de-N-acetylase and methods for using the same

The present invention relates to compositions and methods of their production and use, including use in increasing de-N-acetyl sialic acid antigen of a mammalian cell and methods that exploit the increase in deNAc sialic acid antigen on such cells.

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Oakland, CA, United States of America

Brent T Hagen