Cargill Inc.

Brent Howard Hilbert

Anil Bhagwan Khare

Paula M Hicks

Sara C McFarlan

Fernando A Sanchez-Riera

Christopher Solheid

Kent Floy

Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

Methods and systems for increasing production of equilibrium reactions

The invention provides compositions and methods for isolating phenolic compounds, particularly isoflavones, from aqueous extracts of plant materials that contain such compounds. The process comprising:

Process for isolating phenolic compounds

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South Haven, MN, United States of America

Brent Howard Hilbert