Codexis, Inc.

Hughes, Gregory

Janey, Jacob

Truppo, Matthew

Beutner, Gregory

Grau, Brendan

Ashley, Eric

Limanto, John

Novick, Scott

Mock, Marissa

Strotman, Hallena

Gohel, Anupam

Klapars, Artis

Liang, Jack

Collier, Steven J.

Brendan T Grau

Emily Mundorff

Gregory Hughes

John Limanto

Christopher K Savile

Paul N Devine

Fred J Fleitz

Steven James Collier

Derek J Smith

Jack S Liang

Jacob M Janey

Scott J Novick

Fabien Louis Cabirol

Anupam Gohel

Hallena Strotman

Artis Klapars

Gregory L Beutner

Matthew Truppo

Eric Ashley

Marissa Mock

Hallena R Strotman

Steven J Collier

Fabien L Cabirol

Jack Liang

Gregory Beutner

The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.

Transaminase reactions

The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker.

Biocatalysts and methods for the synthesis of (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine

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Warrington, PA, United States of America

Brendan T Grau