Wisconsin Alumni Research Foundation

Nanyang Technological University

Brendan Patrick Orner

James A Thomson

Laura Lee Kiessling

Ratmir Derda

Vee Vee Cheong

Shu Wen Chew

Rongli Fan

The present invention is directed to a method for fabricating a gold nanoparticle, the method comprising the steps of contacting a gold ion with a protein, wherein the protein has an inner cavity that can accommodate the gold ion, separating the protein with the encapsulated gold ion(s) from non-encapsulated gold ions, contacting the protein-encapsulated gold ion with a first reductant to reduce the gold ion and form a gold nanocluster seed in the inner cavity of the protein, wherein the first reductant is a strong reductant, and contacting the gold nanocluster seed in the inner cavity of the protein with a second reductant to mineralize and grow the gold nanoparticle.

Method for fabricating a gold nanoparticle

A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides.

Defined surfaces of self-assembled monolayers and stem cells

Growing community of inventors

Singapore, Singapore

Brendan Patrick Orner