Monsanto Technology LLC

Dekalb Genetics Corporation

Brenda A Lowe

Jyoti R Rout

Michael T Mann

Brandon Davis

Lubomyr Kucher

Michael Spencer

Whitney R Adams, Jr

David R Duncan

Anisha Akula

William L Petersen

David D Songstad

Wanggen Zhang

Joel B Wilks

Brian J Martinell

Paul S Chomet

John P Purcell

Anne Spelletich

Melissa Way

Whitney Adams

John A Korte

Albert P Kausch

T Michael Spencer

A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.

Methods for producing transgenic plants

The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

Method and apparatus for substantially isolating plant tissues

The present invention discloses and claims methods and devices for the rapid mechanical isolation of monocot plant tissues suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating target plant tissues for use as transformable explants, and propagation of transgenic plants and plant tissues.

Production of transformed plants is achieved by placing-inoculated explants directly onto a medium containing selective agents for suppressing growth ofand killing non-transformed explant cells.

The current invention provides methods and compositions for producing an elite line ofenhanced for transformability. Compositions comprising an elite germplasm exhibiting increased transformability are disclosed. Further provided are methods for breeding maize such that enhanced transformability traits may be transferred to a desired germplasm. The plants and methods of the invention represent a valuable new tool for the creation of elite transgenic plants, preferably having one or more added beneficial characteristics.

Methods and compositions for production of maize lines with increased transformability

The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.

Methods for rapidly transforming monocots

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Mystic, CT, United States of America

Brenda A Lowe