Other

Branko Kozulic

Urs Heimgartner

Klaus Mosbach

Slobodan Barbaric

A method for detecting a glycoprotein using a solid support is disclosed where the glycoprotein is oxidized by periodate, polyacrylic polyhydrazide which is a copolymer having repeating units possessing a hydrazide group and repeating units possessing hydroxyl groups is coupled to the oxidized glycoprotein and a glycoenzyme or radioactive compound containing aldehyde groups or activated ketone groups is coupled to the polyacrylic polyhydrazide which allows for detection of the glycoprotein. The glycoprotein may be directly attached to the solid support or may be bound to an antigen which is immobilized on the solid support.

Polyacrylic hydrazides and their applications as glycoprotein reagents

An acrylic monomer which is an amino sugar alcohol whose primary or secondary amino group has been derivatized by an acryloyl or a methacryloyl function. The amino group can be linked to any carbon of five or six-carbon sugar alcohols. The acrylic monomer has the formula: ##STR1## where R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;

Hydrophilic and amphiphatic monomers, their polymers and gels and

A process for the preparation of stabilized glycoproteins without the introduction of foreign molecules. Susceptible monosaccharides covalently attached to the protein part of the molecule undergo periodate oxidation and, after elimination of the remaining periodate, the oxidized glycoprotein is incubated in a buffer under conditions favorable for the reaction between the aldehyde groups generated in the sugar part and the amino acid residues from the protein part. The oxidized carbohydrate chains act as a polyaldehyde crosslinker, with the cross-linking reaction producing intramolecularly and intermolecularly linked derivatives. The amount and size of the intermolecularly linked derivatives are controlled by degree of oxidation and protein concentration. The thermal stability, depends on the degree of oxidation and under optimal conditions is about 10 times better than the stability of native invertase. Additional stabilization of less than optimally oxidized invertase can be achieved by reduction with NaCNBH.sub.3.

Stabilization of glycoproteins

An electrophoretic method of resolving mixtures of molecules by putting the molecules in an ionic form; contracting them with a porous gel made up of a polymer comprising units derived from a monomer of the formula: ##STR1## wherein R.sub.1 is H, CH.sub.2 OH or (CHOH).sub.m CH.sub.2 OH, m being 1 or 2;

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Zurich, Switzerland

Branko Kozulic