Dow Agrosciences LLC

Agrigenetics, Inc.

Brandon Rapier

Thomas G Patterson

Juan Pablo Raimondi

Jennifer Hamilton

Ana Paula Canu

Christof Stoll

Amy Pierce

Carol Powers

Nitigna Heath

This disclosure concerns the isolation of nucleic acids (e.g., genomic DNA) from plant seed material that has been defatted. In some embodiments, such nucleic acids are of sufficient quality and abundance that they may be used in an amplification-based genetic analysis technique; for example and without limitation, to make selections in a plant breeding program.

Recovery of genomic DNA from remnant extracted seed samples

Methods for separating pericarp tissue from surrounding tissues in grain are provided. Included are methods for isolation of high-purity pericarp DNA from a grain plant that reflects the genotype of the maternal parent of the grain plant, such that the isolated DNA may be used in a PCR-based genotyping assay.

Pericarp DNA extraction and matrilineage determination

Growing community of inventors

Zionsville, IN, United States of America

Brandon Rapier