USA As Represented by Secretary of the Navy

Bradley R Ringeisen

Peter K Wu

Justin C Biffinger

Douglas B Chrisey

Lisa A Fitzgerald

Alberto Pique

Barry J Spargo

Jason Barron

David B Krizman

R Andrew McGill

Raymond C Y Auyeung

Michael Paul Malito

Heungsoo Kim

Russell Kirk Pirlo

Henry Daniel Young

Marlene M Darfler

Kenneth H Nealson

Huey-Daw Wu

James M Fitz-Gerald

Erinn C Howard

Cherie Ziemer

Kurt Henry

Erinn C (Howard) Pirlo

Meghann Ribbens

Christina M Othon

Dana D Dlott

Rohit Modi

A method for printing materials by: providing a receiving substrate; providing a target substrate having a photon-transparent support, a photon absorbent interlayer coated on the support, and a transfer material of a solid-phase environmental sample coated on top of the interlayer opposite to the support; and directing photon energy through the transparent support so that the photon energy strikes the interlayer is described. The environmental sample includes living organisms. A portion of the interlayer is energized by absorption of the photon energy, and the energized interlayer causes a transfer of a portion of the environmental sample including the microorganisms across a gap between the target substrate and the receiving substrate and onto the receiving substrate.

Isolation of microniches from solid-phase and solid suspension in liquid phase microbiomes using laser induced forward transfer

A method using a receiving substrate and a target substrate having a photon-transparent support, a photon absorbent interlayer coated on the support, and a tissue-implantable particle on top of the interlayer opposite to the support. A source of photon energy is directed through the transparent support so that the photon energy strikes the interlayer. A portion of the interlayer is energized by absorption of the photon energy. The energized interlayer causes a transfer of the particle across a gap between the target substrate and the receiving substrate and embedding of the particle into the receiving substrate.

Laser induced ballistic particle implantation technique

Disclosed is an apparatus having: a pressure chamber and a gas-producing microorganism within the chamber. The pressure chamber is capable of maintaining a gas pressure of at least 0.5 psi above atmospheric pressure.

Bacteria-based gas generator

Disclosed herein is a structure having: a porous polymeric film permeated by a first extracellular matrix material; and a topcoat layer comprising a second extracellular matrix gel disposed on the film. Also disclosed herein is a method of: providing a porous polymeric film; permeating the film with a first extracellular matrix material; and applying a topcoat layer of a second extracellular matrix material to the film. Also disclosed herein is a method of: laser-machining holes through a film comprising collagen to form a web-like structure.

Cell and biofactor printable biopapers

A device having: a chamber having a gas inlet, a gas vent, and a liquid vent; and a float and a weight coupled to the chamber. The float has a lower density than the chamber. The weight has a higher density than the chamber. The aggregate density of the chamber, the float, and the weight is greater than the density of the chamber. The gas inlet, the gas vent, the liquid vent, the float, and the weight are positioned on the chamber such that: when the chamber is filled with and submerged in a liquid in which the chamber is neutrally-buoyant, the chamber is oriented to place the gas vent below the gas inlet; and when a gas is introduced through the gas inlet into the chamber that is filled with the liquid, the chamber pivots to raise the gas vent until a portion of the gas escapes from the chamber through only the gas vent.

Bouyancy control device

A method of: providing a mixture of fecal waste and a bacterium; incubating the mixture to produce a fatty acid enriched mixture; removing water from the fatty acid enriched mixture to produce a dried mixture; and pyrolyzing the dried mixture in an inert atmosphere to produce an alkane from the C-Cfatty acid. The bacterium is a type that produces a C-Cfatty acid in the presence of any microbes in the fecal waste.

Alkane enhancement of waste using microbial pre-treatement

A high throughput biological screening assay comprising at least two anodes, at least two cathodes acting as the reference electrode, and a polymer membrane placed between each anode and cathode, wherein the at least two anodes comprise a biological culture, and wherein the at least two cathodes comprise an oxidizing agent and a buffering agent. The high throughput biological screening assay wherein the at least two cathodes are connected in parallel to simulate the connection between the same cathode and different anodes. The high throughput biological screening assay further including an external resistor or open circuit and means for measuring the voltage across the external resistor or open circuit. A method of measuring power generation using a single cathode as a reference electrode to monitor the biological production of energy. A method of correlating bacterial biofilm formation within an operational microbial fuel cell directly to current output.

High-throughput biological screening assay using voltage gradients

A method of directing a pulse of laser energy though a workpiece. The workpiece has: a substrate that transmits the laser energy; focusing elements on a surface of the substrate proximal to the laser that focus the laser energy; and a coating on the substrate distal to the laser that absorbs a portion of the laser energy. Each focusing element focuses the laser energy to a point that removes or ablates a portion of the coating from the substrate to produce a hole in the coating.

Sub-micron laser direct write

A fuel cell comprising an anode chamber, a cathode chamber, and a nanoporous membrane between the anode chamber and the cathode chamber, wherein the nanoporous membrane sequesters and isolates a microbe in the anode chamber. The nanoporous membrane allows nutrients to flow actively or passively from the cathode chamber to the anode chamber and can be modified by a thin film composite (TFC) to create a TFC nanofiltration membrane. The nanoporous membrane can have a pore size from about 100 nm to about 1000 nm. A method of making a fuel cell comprising configuring a nanoporous membrane between an anode chamber and a cathode chamber wherein the nanoporous membrane sequesters and isolates a microbe in the anode chamber and can be used to protect the cathode chamber.

Biological fuel cells with nanoporous membranes

A method of laser forward transfer is disclosed. Photon energy is directed through a photon-transparent support and absorbed by a polymer interlayer coated thereon. The energized interlayer causes the transfer of a biological material coated thereon across a gap and onto a receiving substrate.

Biological laser printing via indirect photon-biomaterial interactions

A method of laser forward transfer is disclosed. Photo energy is directed through a photon-transparent support and absorbed by an interlayer coated thereon. The energized interlayer causes the transfer of specific regions of a heterogeneous tissue sample coated thereon across a gap and onto a receiving substrate or into a receiving vessel.

Biological laser printing for tissue microdissection via indirect photon-biomaterial interactions

A method of laser forward transfer is disclosed. Photon energy is directed through a photon-transparent support and absorbed by an interlayer coated thereon. The energized interlayer causes the transfer of a biological material coated thereon across a gap and onto a receiving substrate.

A method for creating a microarray of biomaterial uses a source of laser energy, a receiving substrate, and a target substrate. The target substrate comprises a laser-transparent support having a laser-facing surface and a support surface. The target substrate also comprises a composite material having a back surface in contact with the support surface and a front surface. The composite material comprises a mixture of the biomaterial to be deposited and a matrix material. The matrix material is a material that has the property that, when it is exposed to laser energy, it desorbs from the laser-transparent support. The source of laser energy is positioned in relation to the target substrate so that laser energy is directed through the laser-facing surface of the target substrate and through the laser-transparent support to strike the composite material at a defined target location. The receiving substrate is positioned in a spaced relation to the target substrate. The source of laser energy has sufficient energy to desorb the composite material at the defined target location, causing the composite material to desorb from the defined target location and be lifted from the support surface of the laser-transparent support. The composite material is deposited at a defined receiving location on the receiving substrate. The steps are repeated at successive defined target locations and successive defined receiving locations such that the composite material is deposited in a microarray of deposited composite material. The method is useful for creating, for example, a gene recognition array,

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Lorton, VA, United States of America

Bradley R Ringeisen