University of Oklahoma

Bradley P Kropp

Earl Y Cheng

Hsueh-Kung Lin

Yuan Yuan Zhang

Rick Cowan

Brian Grady

Fadee Mandelek

Peter Moore

A tissue graft composition is described herein that includes a segment of small intestinal submucosa having at least one nanoparticle incorporated therein such that the permeability of the segment of small intestinal submucosa is altered, thereby providing the segment of small intestinal submucosa with a more substantially uniform structure for cell migration and proliferation. The tissue graft composition may further comprise at least one macromolecule incorporated into the nanoparticle. The tissue graft composition may be utilized in seeded or unseeded methods of tissue repair.

Tissue graft compositions and methods for producing same

A method for providing a urinary tract tissue graft composition includes providing a segment of small intestinal submucosa and isolating and culturing at least one multipotent cell type from a tissue specimen of a subject. The at least one multipotent cell type is then seeded upon a surface of the segment of small intestinal submucosa and allowed to differentiate into a unipotent cell type, thereby forming a urinary tract tissue graft. Methods for repairing a damaged urinary tract tissue of a subject are also disclosed.

Urinary tract tissue graft compositions and methods for producing same

A method for repairing damaged or diseased urinary tract tissue includes providing a urinary tract tissue graft composition that includes a distal ileal segment of small intestinal submucosa. The distal ileal segment of small intestinal submucosa may be utilized as an unseeded tissue graft composition, or the distal ileal segment of small intestinal submucosa may be positioned in a tissue culture frame, and smooth muscle and urothelial cells isolated from a tissue specimen of a subject and cultured are then seeded upon the distal ileal segment of small intestinal submucosa, thereby forming a urinary tract tissue graft.

A method for providing a urinary tract tissue graft composition includes providing a segment of small intestinal submucosa and positioning the segment of small intestinal submucosa in a tissue culture frame such that the segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame. At least one multipotent cell type is isolated from a tissue specimen of a subject and cultured, and then seeded upon a surface of the segment of small intestinal submucosa, thereby forming a urinary tract tissue graft. Methods for repairing a damaged urinary tract tissue of a subject are also disclosed.

A method for providing a urinary tract tissue graft composition includes providing a tissue culture frame and a segment of small intestinal submucosa and positioning the segment of small intestinal submucosa in the tissue culture frame such that the segment of small intestinal submucosa is suspended and held in a taut position by the tissue culture frame. Smooth muscle and urothelial cells are isolated from a tissue specimen of a subject and cultured, and then seeded upon the segment of small intestinal submucosa, thereby forming a urinary tract tissue graft. A tissue culture frame in which such a urinary tract tissue graft may be formed is also disclosed.

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Edmond, OK, United States of America

Bradley P Kropp