Wisconsin Alumni Research Foundation

Semba Biosciences, Inc.

Sonoplot, Inc.

Bradley James Larson

Anil Rajaram Oroskar

Max G Lagally

Amit Lal

Ferencz S Denes

Chung Hoon Lee

Sorin Odisei Manolache

Thomas P Blandino

Andrew Paul Wilke

Anthony Charles Grabski

Luis Emilio Cruz-Barba

Joerg Werner Baier

David M Barnes

Jason M Helgren

Herbert Edmund Paaren

Martha M Vestling

Thomas Paul Blandino

A device and method for the detection of the interaction between two or more objects is disclosed. The device utilizes electrical impedance spectra measured from a piezoelectric element attached to one such object. Comparison of such spectra along a range of traverse allows precise estimates of distance to be made. One useful application is the topographical mapping of a surface.

Device for detecting interaction with an object

The present invention provides devices and methods for micro-scale simulated moving bed chromatography (SMB) for continuous preparation of analytic quantities of highly pure fractions of target molecules. The present apparatus and method of the invention is adapted in a preferred embodiment to separations by affinity chromatography involving three discontinuous liquid flow loops. An alternative embodiment of affinity chromatography utilizes standard SMB operating under isocratic conditions.

Control system for simulated moving bed chromatography

Methods for producing plasma-treated, functionalized inorganic oxide surfaces are provided. The methods include the steps of subjecting an oxide surface to a plasma to create hydroxyl functionalities on the surface and reacting the hydroxyl functionalities with epoxy group-containing molecules in situ in the absence of plasma. Biomolecules may be immobilized on the resulting functionalized surfaces. The methods may be used to treat a variety of oxide surfaces, including glass, quartz, silica and metal oxides.

Plasma-enhanced functionalization of inorganic oxide surfaces

Devices and methods for depositing fluids on substrates in patterns of spots, lines, or other features use a nozzle, which is preferably configured similarly to a micropipette, having a piezoelectric crystal or other ultrasonic actuator coupled to one of its sides. The nozzle may be charged via capillary action by dipping it into a well containing the fluid to be deposited, and may then be positioned over a desired area of a substrate, at which point activation of the ultrasonic actuator at ultrasonic frequencies will eject the fluid onto the substrate. The needle may subsequently be dipped into a well of rinsing fluid for cleaning. Spots or lines on the order of 5 micrometers width may be generated, making the invention particularly suitable for use in biological applications such as microarray production and in microelectronics applications such as the printing of organic circuitry.

Methods and apparata for precisely dispensing microvolumes of fluids

Methods for producing plasma-treated, functionalized carbon-containing surfaces are provided. The methods include the steps of subjecting a carbon-containing substrate to a plasma to create surface active sites on the surface of the substrate and reacting the surface active sites with stable spacer molecules in the absence of plasma. Biomolecules may be immobilized on the resulting functionalized surfaces. The methods may be used to treat a variety of carbon-containing substrates, including polymeric surfaces, diamond-like carbon films and carbon nanotubes and nanoparticles.

Plasma-enhanced functionalization of carbon-containing substrates

The present invention relates to small sample spots for MALDI targets and methods for producing the same. The sample spots are composed of a layer of matrix material and a layer of analyte. In some instances, the samples spots have diameters of no more than 50 micrometers or even smaller. The sample spots are deposited onto a MALDI target substrate using ultrasonic deposition from a nozzle.

Deposition of samples and sample matrix for enhancing the sensitivity of matrix assisted laser desorption/ionization mass spectrometry

Growing community of inventors

Madison, WI, United States of America

Bradley James Larson