Cellular Dynamics International, Inc.

Fujifilm Cellular Dynamics, Inc.

Brad Swanson

Wen Bo Wang

Lucas Chase

Chris McMahon

Matt George

Carrie Chavez

Nathan Meyer

Steven James Kattman

Steve Fiene

Chad Koonce

Natsuyo Aoyama

Jennifer Luebke-Wheeler

Methods are provided for efficient production of midbrain dopaminergic (DA) neurons. In some aspects, methods involve differentiation and selection of DA neurons for a transgenic pluripotent cell population (e.g., cells comprising a selectable marker gene). Cell populations produced by the instant methods and methods of their use are likewise provided.

Production of midbrain dopaminergic neurons and methods for the use thereof

Methods and composition for maintenance of cardiomyocytes are provided. For example, in certain aspects methods including culturing the cardiomyocytes in a medium essentially free of serum or containing dialyzed serum to maintain long-term purity. In further aspects, methods for modulation of cardiomyocytes may be provided.

Cardiomyocyte medium with dialyzed serum

The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example.

Metabolic maturation in stem cell-derived tissue cells

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Madison, WI, United States of America

Brad Swanson