The Uab Research Foundation

Sierra Lobo, Inc.

Brad Stoops

Arnon Chait

Lawrence James DeLucas

Mark Steven Haberbusch

Alexander I Belgovskiy

Robert J Stochl

Martin E Roth

Chinh T Nguyen

A storage system useful for storing cryogenic liquids is disclosed. The storage system includes a storage vessel, insulation surrounding the storage vessel and a flexible vacuum jacket enclosing them both. The flexible vacuum jacket can be made from a flexible plastic film such as polyimide. Polyimide is preferred due to its ability to remain flexible at cryogenic temperatures. The inner volume of the flexible vacuum jacket is placed under vacuum such that the insulation is maintained under vacuum. The flexible vacuum jacket can collapse against and conform to the insulation when it is evacuated, saving substantially on volume. A fitting for combining multiple passageways is also disclosed, so that multiple passageways can proceed through the vacuum jacket through a single penetration.

Storage system having flexible vacuum jacket

A method of screening solution conditions suitable for the crystallization of macromolecules with a picogram to a microgram of protein using picoliter or nanoliter volumes is provided. A preferred method comprises preparing a plurality of recipe solutions in milliliter volumes, aspirating protein and recipe solutions, respectively, from a plurality of wells, and dispensing the protein and recipe solutions into a plurality of wells, covering the combined protein and recipe solutions with oil, and maintaining the solution wells until crystallization or precipitation occur therein.

Method for preparation of microarrays for screening of crystal growth conditions

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Bay Village, OH, United States of America

Brad Stoops